Analiza flavonoida i antimikrobna aktivnost komercijalno dostupnih proizvoda od propolisa

Scientific paper - Original scientific paper

• Title (english): Flavonoid analysis and antimicrobial activity of commercially available propolis products
• Author: Ivan Kosalec
• Author: Stjepan Pepeljnjak
• Author: Marina Bakmaz
• Author: Sanda Vladimir-Knežević
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy)
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of microbiology)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract (croatian)

Etanolne iscprine propolisa najčešće su korišteni proizvodi od propolisa na tržištu za liječenje manjih vrijedova u usnoj šupljini, angine, mlječca i kožnih infekcija. Zbog toga što je propolis još uvijek neoficijelna droga u ljekarništvu, ispitali smo sadržaj flavonoida u deset komercijalno dostupnih etanolnih iscrpina propolisa na hrvatskom tržištu koristeći dvije komplementarne spektrofotometrijske metode. Antimikrobna aktivnost
uspoređena je primjenom difuzionog testa na šest bakterijskih vrsta – Bacillus subtilis NCTC 8236, Staphylococcus aureus ATCC 25923, Streptococcus pyogens ATCC 12204, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 10536, Pseudomonas aeruginosa ATCC
27853 i jednu kvascima sličnu gljivičnu vrstu – Candida albicans ATCC 10231. Rezultati sadržaja flavonoida pokazuju da je sadržaj flavona i flavonola u proizvodima ujednačen (od 0,14 do 0,41%), međutim sadržaj flavanona jako varira između 0,43 i 18,78%. Ukupni
sadržaj flavonoida prikazan kao zbroj dviju komplementarnih metoda u proizvodima od propolisa je između 0,78 i 18,92%, a većina proizvoda sadrže flavonoide ispod 9%. Svi proizvodi sa sadržajem ukupnih flavonoida višim od 1% pokazuju antimikrobnu aktivnost protiv četiri gram-pozitivne bakterijske vrste, na vrstu Pseudomonas aeruginosa i kvascima sličnu gljivičnu vrstu Candida albicans. Ukupni sadržaj flavonoida prikazan kao zbroj dviju spektrofotometrijskih metoda koristan je podatak za procjenu sadržaja flavonoida u proizvodima od propolisa. Naši rezultati ukazuju na potrebu analize kakvoće proizvoda od propolisa.

Abstract (english)

Propolis ethanolic solutions are the most used propolis products on the market for the treatment of minor ulcers in the mouth, angina, thrush or skin infections. Since it is still unofficial drug in pharmacy, we analyzed the contents of flavonoids in ten commercially available ethanolic solutions of propolis from the Croatian market using two complementary colorimetric methods. Antimicrobial activity, determined with the diffusion method, against six bacterial species (Bacillus subtilis NCTC 8236, Staphylococcus aureus ATCC 25923, Streptococcus pyogenes ATCC 12204, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 10536, Pseudomonas aeruginosa ATCC 27853, and one yeast-like fungi Candida albicans ATCC 10231 were compared. Results of flavonoids analysis suggested that the contents of flavones and flavonols in the products were uniform and ranged from 0.14 to 0.41%, but the content of flavanones varied greatly from 0.43 to 18.78%. Total flavonoid content, as the sum of two colorimetric methods, in propolis products was between 0.78 and 18.92%, and the most products had flavonoids content below 9%. All products with the total flavonoids content above 1% showed antimicrobial activity against the four Gram-positive bacterial species tested, and against P. aeruginosa and the yeast-like fungus C. albicans. Total flavonoid contents, expressed as the sum of two colorimetric methods, could be useful methods for estimating the flavonoid contents of propolis products. Our results indicate that the quality of commercially available propolis products requires verification.

• Keywords (croatian): propolis; proizvodi; flavonoidi; antimikrobno djelovanje; spektrofotometrija
• Keywords (english): propolis; products; flavonoids; antimicrobial; colorimetric
• Language: croatian
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review - international
• Publication version: Published version
• Journal title: Acta Pharmaceutica
• Numbering: vol. 55, no. 4, pp. 423-430
• p-ISSN: 1330-0075
• e-ISSN: 1846-9558
• URN:NBN: urn:nbn:hr:163:820377
• Publication: 2005
• Document URL: https://hrcak.srce.hr/19048
• Type of resource: Text
• Access conditions: Open access
• Created on: 2020-04-08 16:46:09