Rhamnus intermedia Steud. et Hochst. – a new source of bioactive phytochemicals

Scientific paper - Preliminary note / Short communication

• Author: Dunja Šamec
• Author: Dario Kremer
• Author: Jiří Grúz
• Author: Renata Jurišić Grubešić
• Author: Jasenka Piljac-Žegarac
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical analysis)
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical botany)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract (english)

Antioxidant capacity and various classes of phenolic antioxidants were quantified in leaf and
bark methanol extracts of a medicinal plant, Rhamnus intermedia Steud. et Hochst., whose phytochemical
composition is unknown. Three well-established assays were used to determine the antioxidant potency of
the extracts: the Ferric Reducing/Antioxidant Power assay (FRAP), 2,2-diphenyl-1-picrylhydrazyl
(DPPH) assay, and 2,2'-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) assay, as well as oxygen
radical absorbance capacity (ORAC) assay. Total phenol (TP, Folin–Ciocalteu assay), total flavonoid
(TF, colorimetric assay with AlCl3) and free phenolic acid (UPLC-MS/MS) content of extracts was quantified.
In comparison to bark extracts, leaf extracts exhibited significantly higher TP and TF contents, as
well as higher antioxidant capacity according to all four assays. Ten free phenolic acids were quantified in
leaf extracts while six were detected in bark extracts. The most abundant phenolic acid in leaves was
vanilic acid (1647.06 ± 79.35 μmol g–1 DW; DW, dry weight) while salycilic acid was the most concentrated
in the bark (111.10 ± 14.14 μmol g–1 DW). (doi: 10.5562/cca1946)

• Keywords (english): Rhamnus intermedia; antioxidant activity; total phenols; total flavonoids; phenolic acids
• Language: english
• Publication type: Scientific paper - Preliminary note / Short communication
• Publication status: Published
• Peer review: Peer review - international
• Publication version: Published version
• Journal title: Croatica Chemica Acta
• Numbering: vol. 85, no. 2, pp. 125-129
• p-ISSN: 0011-1643
• e-ISSN: 1334-417X
• DOI: https://doi.org/10.5562/cca1946
• URN:NBN: urn:nbn:hr:163:150461
• Publication: 2012
• Document URL: https://hrcak.srce.hr/84520
• Type of resource: Text
• Access conditions: Open access
• Created on: 2020-04-17 14:15:14