Naringenin reducira histopatološke promjene u jetri i bubregu miševa s dijabetesom

Scientific paper - Original scientific paper

• Title (english): Naringenin ameliorates pathological changes in liver and kidney of diabetic mice: a preliminary study
• Author: Damir Sirovina
• Author: Nada Oršolić
• Author: Marijana Zovko Končić
• Author: Gordana Gregorović
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract (croatian)

Istražen je učinak naringenina, flavonoida dostupnoga iz grejpa, naranče i rajčice, na razinu lipidne peroksidacije te patohistološke promjene u jetri i bubregu miševa s dijabetesom prouzročenim aloksanom. Dva dana nakon injekcije aloksana (75 mg kg-1, iv) započeta je terapija etanolnom otopinom naringenina (0.5% v / v). Naringenin je injiciran miševima intraperitonealno u dozi od 50 mg kg-1 tijekom sedam dana. Učinak naringenina na razinu lipidne peroksidacije određen je metodom s tiobarbiturnom kiselinom, a histopatološke promjene jetre i bubrega utvrđene su svjetlosnim mikroskopom. Terapija naringeninom prouzročila je značajno smanjenje ne samo razine lipidne peroksidacije u tkivu jetre i bubrega nego i broja vakuoliziranih stanica jetre te stupnja njihove vakuolizacije. Naznake oporavka tkiva u bubregu navode na zaključak da bi dugotrajna terapija mogla smanjiti oštećenja bubrega prouzročena dijabetesom. Zaštitni učinak naringenina u razvoju dijabetičkih komplikacija u miševa daje nadu da je sličan, pozitivan učinak moguć i u ljudi.

Abstract (english)

The effect of naringenin, a flavonoid found in grapefruit, orange, and tomato, on lipid peroxidation and histopathological changes in the liver and kidneys of alloxan-induced diabetic mice were investigated. Two days after alloxan injection (75 mg kg-1, i.v.), naringenin ethanolic solution (0.5 % v/v) was given to mice intraperitoneally (50 mg kg-1 per day) for seven days. Naringenin’s impact on lipid peroxidation was measured by the 2-thiobarbituric acid test and histopathological changes were examined under a light microscope. Naringenin administration resulted in a significant decrease of lipid peroxidation level in liver and kidney tissue, as well as in a decreased number of vacuolated liver cells and degree of vacuolisation. Indications of tissue repair in kidney suggested that amelioration of diabetes-induced renal damage could be achieved over a longer period of time. Findings suggest that naringenin could be considered a dietary supplement in the prevention or treatment of diabetic complications and other diseases connected wih oxidative stress, and gives a hope that it could show similar effects in the treatment of diabetes in humans.

• Keywords (croatian): dijabetes; dijabetička nefropatija; histopatologija; lipidna peroksidacija; naringenin; oštećenja jetre
• Keywords (english): diabetes; diabetic kidney; histopathology; lipid peroxidation; liver disease; naringenin
• Language: croatian
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review - international
• Publication version: Published version
• Journal title: Arhiv za higijenu rada i toksikologiju
• Numbering: vol. 67, no. 1, pp. 19-24
• p-ISSN: 0004-1254
• e-ISSN: 1848-6312
• DOI: https://doi.org/10.1515/aiht-2016-67-2708
• URN:NBN: urn:nbn:hr:163:182999
• Publication: 2016
• Document URL: https://hrcak.srce.hr/154583
• Type of resource: Text
• Access conditions: Open access
• Created on: 2020-05-14 15:55:26