HPLC analysis of citric and tartaric acids in fruit nectars and juices

Scientific paper - Original scientific paper

• Author: Suzana Inić
• Author: Maja Ljepović
• Author: Ana-Marija Domijan
• Author: Sabina Steiner Srdarević
• Author: Jasna Jablan
• Author: Ksenija Markov
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of analytical chemistry)
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical botany)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract (english)

The aim of this study was to estimate a level of citric and tartaric acids in fruit nectars (n = 17) and juices (n = 13) available on the Croatian market. For analysis reverse-phase HPLC with UV/Vis detector set at 214 nm was used. Mobile phase was phosphate buffer (50 mM, pH = 2.80) at flow rate 0.5 mL min–1. The method was linear (r2 = 0.9999). LoD was 0.01 g L–1, LoQ was 0.03 g L–1 and the intra-day along with the inter-day variability were up to 3 %. The level of citric and tartaric acids in fruit nectars ranged from 1.26 to 4.42 g L–1 and 0.68 to 0.86 g L–1, respectively, and in fruit juices ranged from 3.03 to 7.67 g L–1 and 3.09 to 4.68 g L–1, respectively. A higher level of citric acid in fruit juices than in fruit nectars was detected (p < 0.05; MannWhitney U test). Six fruit juices contained a higher level of citric acid allowed by EU regulation implying the importance of monitoring concentrations of both acids in food products.
<br><a rel="license" href="http://creativecommons.org/licenses/by/4.0/"><img alt="Creative Commons License" style="border-width:0" src="https://i.creativecommons.org/l/by/4.0/80x15.png" /></a> This work is licensed under a <a rel="license" href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution 4.0 International License</a>.

• Keywords (english): citric acid; tartaric acid; fruit juices; monitoring; green chemistry; Croatian market
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review - international
• Publication version: Published version
• Journal title: Croatica Chemica Acta
• Numbering: vol. 93, no. 1, pp. 57-62
• p-ISSN: 0011-1643
• e-ISSN: 1334-417X
• DOI: https://doi.org/10.5562/cca3662
• URN:NBN: urn:nbn:hr:163:433369
• Publication: 2020
• Document URL: https://hrcak.srce.hr/244449
• Type of resource: Text
• Access conditions: Open access
• Created on: 2020-11-17 14:28:39