Prevalidacija CUPRAC metode za određivanje ukupnog antioksidativnog kapaciteta

master's thesis

• Title (english): Prevalidation of the CUPRAC method for determination of total antioxidant capacity
• Author: Mateja Bainac
• Mentor: Jadranka Vuković Rodriguez (mentor)
• Committee member: Jadranka Vuković Rodriguez (predsjednik povjerenstva)
• Committee member: Renata Jurišić Grubešić (član povjerenstva)
• Committee member: Živka Juričić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical analysis); Zagreb
• Defense date and country: 2016-07-08, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

U okviru ovog diplomskog rada provedena je prosudba validacijskih značajki i kontrola kakvoće CUPRAC
metode za određivanje ukupnog antioksidativnog kapaciteta te je primjenom ispitanog postupka određen
ukupni antioksidativni kapacitet vodenog i metanolnog ekstrakta Yunnan Baiyao praha. CUPRAC metoda
temelji se na mjerenju apsorbancije CUPRAC kromofora, Cu(I)- neokuproin (Nc) kelata, koji se formira
kao produkt redoks reakcije antioksidansa s CUPRAC reagensom, bis(neokuproin)bakar(II) kationom
[Cu(II)-Nc] na 450 nm. Evaluacija i kontrola valjanosti CUPRAC metode provedena je primjenom
cijelovite prevalidacijske strategije prema Grdinić i Vuković (2004). Prevalidacijski postupak uključuje
karakterizaciju skupina od 1 do 6, ispitivanje homogenosti, ispitivanje odnosa analit-signal, prepoznavanje
bjegunaca i prosudbu graničnih vrijednosti. Izvedbene karakteristike pokazale su idealnu linearnu
kalibracijsku funkciju ( Sˆ = 0,002 x), idealnu analitičku prosudbenu funkciju ( xˆ = 525,6 S), vrlo nisku
granicu dokazivanja (LD= 16,03 μM), nepovoljnu granicu određivanja (LQ=97,46 μM) te nižu preciznost (
rx s ˆ od 1,54- 30,69%) i točnost (Δx od 1,74-16,21 %). Provedena prevalidacija ukazala je na nedostatke
postupka kao što je nehomogenost slijepih vrijednosti, što utječe na cjelokupnu kakvoću postupka.
Antioksidativni kapacitet vodenog i metanolnog ekstrakta Yunnan Baiyao praha izražen je u μg TE/g praha.
Utvrđeno je kako vodeni ekstrakt Yunnan Baiyao praha ima veći antioksidativni kapacitet ( 897,2 ± 51,5) u
odnosu na metanolni ekstrakt (702,3 ± 68,0).

Abstract (english)

In this paper the evaluation of validation characteristics and quality control of the CUPRAC method was
carried out and using the tested procedure total antioxidant capacity of aqueous and methanol extracts of
Yunnan Baiyao powder was determined. The CUPRAC method is based on the absorbance measurement of
the CUPRAC chromophore, Cu(I)-neocuproine (Nc) chelate, formed as a result of the redox reaction of
antioxidants with the CUPRAC reagent, bis(neocuproine)copper(II) cation [Cu(II)-Nc] at 450 nm.
Evaluation and quality control of the CUPRAC method was carried out using a comprehensive
prevalidation strategy according to Grdinić and Vuković (2004). The prevalidation procedure included
characterization of the groups from 1 to 6, checking of limiting groups, homogenity testing, the quality of
analyte-signal relationship, outlier recognition, and evaluation of limiting values. Performance
characteristics showed an ideal linear calibration function ( Sˆ = 0,002 x), an ideal analytical evaluation
function ( xˆ = 525,6 S), a very low limit of detection (LD= 16,03 μM), a somewhat poorer limit of
quantitation (LQ=97,46 μM) and a lower precision ( rx s ˆ 1,54- 30,69%) and accuracy (Δx 1,74-16,21 %).
The carried out prevalidation also indicated disadvantages of the procedures such as inhomogeneity of
blank values, which influences the total quality of procedure. The total antioxidant capacity of aqueous and
methanol extracts of Yunnan Baiyao powder is expressed as μg TE/g powder. It has been determined that
the aqueous extract of Yunnan Baiyao powder has a higher antioxidant capacity ( 897,2 ± 51,5) than the
methanol extract (702,3 ± 68,0).

• Keywords: prevalidacija; ukupni antioksidativni kapacitet; CUPRAC metoda; Yunnan Baiyao
• Keywords (english): prevalidation; total antioxidant capacity; CUPRAC method; Yunnan Baiyao
• Language: croatian
• URN:NBN: urn:nbn:hr:163:368167
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2016-12-06 14:31:23