Učestalost genotipova ABO sustava krvnih grupa u oboljelih od kolorektalnog karcinoma

master's thesis

• Title (english): Distribution of ABO genotypes among patients with colorectal cancer
• Author: Pavica Šonjić
• Mentor: Karmela Barišić (mentor)
• Committee member: Karmela Barišić (predsjednik povjerenstva)
• Committee member: Nada Vrkić (član povjerenstva)
• Committee member: Jasna Bingulac-Popović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of medical biochemistry and haematology); Zagreb
• Defense date and country: 2018-02-28, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Medical Biochemistry

Abstract

Kolorektalni karcinom jedan je od pet najčešći karcinoma u svjetskoj i hrvatskoj populaciji. Kako bi se omogućilo preventivno djelovanje, pridonijelo njegovom ranom otkrivanju i poboljšao ishod liječenja, nužno je naći pouzdane prognostičke i prediktivne pokazatelje kao i čimbenike rizika. Istraživanja povezanosti ABO sustava krvnih grupa s gastrointestinalnim karcinomima pokazuju povezanost ne-O krvnih grupa i povećanog rizika za obolijevanje. Takva istraživanja u slučaju kolorektalnog karcinoma malobrojna su i pokazuju oprečne rezultate. Cilj ovog istraživanja bio je utvrditi povezanost ABO genotipova i rizik od nastanka kolorektalnog karcinoma u hrvatskoj populaciji. Istraživanje je obuhvatilo 80 oboljelih od kolorektalnog karcinoma i kontrolnu skupinu od 303 zdrava dobrovoljna davatelja krvi. Primjenom PCR-SSP metode provedena je genotipizacija na 5 alela (O1, O2, A1, A2, B) i 15 ABO genotipova. Rezultati su podvrgnuti statističkom testiranju pomoću omjera izgleda, χ2 testa i testa razlike proporcija. Testom omjera izgleda nisu dobivene statistički značajne razlike između ABO-genotipova. Rezultati istog testa na fenotipskoj razini pokazali su 1,79 puta povećani rizik za obolijevanje od kolorektalnog karcinoma za krvnu grupu A u odnosu na krvnu grupu O (OR=1,79; 95 % CI=1,01-3,19). Testom razlike proporcija pokazano je da je pojavnost alela O1 statistički značajno veća u kontrolnoj skupini (0,62) u odnosu na skupinu oboljelih (0,53). Dobivena je i granična statistička značajnost prema kojoj je pojavnost alela A2 statistički niža u kontrolnoj skupini (0,035) u odnosu na skupinu oboljelih (0,069). Rezultati ovog istraživanja potvrdili su dosadašnja opažanja u slučaju gastrointestinalnih karcinoma i povezanosti s ABO krvnim grupama te predstavljaju dobru podlogu za genska i in silico istraživanja mehanizama kojima antigeni ABO krvnih grupa pridonose karcinogenezi.

Abstract (english)

Colorectal cancer is one of the most common cancers worldwide and among the Croatian population. To improve colorectal carcinoma prevention, contribute to its early detection and improve the outcome of treatment, it is necessary to find reliable prognostic and predictive factors as well as risk factors. Investigations of the ABO blood group correlation with gastrointestinal carcinoma show the association of non-O blood groups with an increased risk of disease. Such investigations in the case of colorectal cancer are few and show contradictory results. The aim of this study was to examine the association between the ABO genotypes and the risk of colorectal cancer in the Croatian population. The study included 80 patients with colorectal carcinoma and a control group of 303 healthy volunteer blood donors. Using the PCR-SSP method, genotyping was performed on 5 main alleles (O1, O2, A1, A2, B) and 15 ABO genotypes. The results were statistically tested by odds ratio, Chi-square test and an analysis of the proportional difference. No apparent statistically significant difference between ABO genotypes was obtained by odds ratio testing. The results of the same test at the phenotypic level showed 1.79 times the increased risk for colorectal carcinoma of blood type A compared to blood group O (OR=1.79; 95% CI=1.01–3.19). The proportional difference test showed that the incidence of allele O1 was statistically significantly higher in the control group (0.62) compared to the group of patients (0.53). Borderline statistical significance was obtained according to the presence of allele A2 which was statistically lower in the control group (0.035) compared to the group of patients (0.069). The results of this study confirmed the previous major findings due to gastrointestinal carcinoma and association with the ABO blood groups, and represent a good basis for genetic and in silico research of the mechanisms by which the ABO blood groups antigens contribute to carcinogenesis.

• Keywords: ABO krvne grupe; ABO genotipizacija; kolorektalni karcinom; omjer izgleda; test razlike proporcija
• Keywords (english): ABO blood groups; ABO genotyping; colorectal cancer; odds ratio; the proportional difference test
• Language: croatian
• URN:NBN: urn:nbn:hr:163:178433
• Study programme: Title: Medical biochemistry; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra medicinske biokemije (magistar/magistra medicinske biokemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-12-13 11:07:53