Provjera granice kvantifikacije lukocita i trombocita na hematološkom brojaču Sysmex XN-1000

master's thesis

• Title (english): Limit of quantification check for leukocyte and platelet count using Sysmex XN-1000 hematology analyzer
• Author: Domagoj Šebečić
• Mentor: Dunja Rogić (mentor)
• Committee member: Dunja Rogić (predsjednik povjerenstva)
• Committee member: Suzana Inić (član povjerenstva)
• Committee member: Ivana Rako (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of medical biochemistry and haematology); Zagreb
• Defense date and country: 2018-07-09, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Medical Biochemistry

Abstract

Hematološki brojač Sysmex XN-1000 je često korišten i pouzdan analitički uređaj u hematološkim laboratorijima. Cilj istraživanja
bio je provjeriti granice kvantifikacije (LoQ) leukocita u tzv. „low white blood cell“ načinu rada uređaja koji se sve češće koristi,
posebno kod imunosuprimiranih pacijenata, te je laboratorijskom osoblju važno koju dobivenu vrijednost može s pouzdanjem izdati kao točnu i preciznu. Također smo provjerili i granicu kvantifikacije trombocita u uobičajenom načinu... More rada.Za određivanje granice kvantifikacije leukocita koristili smo nativne uzorke s iznimno niskim brojem leukocita (<0,2x10^9/L), te uzorke s višim brojem leukocita (>1x10^9/L) razrijeđene plazmom kako bi razrijeđeni uzorak bio što vjerniji matriksu nativnog uzorka. „Low-WBC“ način rada koristi tzv. WDF kanal, te svako mjerenje izvodi u triplikatu čime se povećava preciznost postupka. Kod razrjeđivanja uzoraka za mjerenja trombocita korištena je plazma dobivena dvostrukim centrifugiranjem na 4000 okretaja/min. Isprva smo odredili razinu signala pozadine (limit of blank), te se uvjerili da ona iznosi 0.00 x10^9/L. Granicu kvantifikacije leukocita dobili smo eksperimentalno, sustavnim mjerenjem sve nižih koncentracija leukocita (1.0, 0.8, 0.5, 0.2, 0.1, 0.09, 0.07, 0.05, 0.03) x10^9/L. Zatim smo uz prethodno dogovoren kriterij za maksimalno prihvatljivu netočnost i preciznost od 10% utvrdili kako je granica kvantifikacije 0.1x10^9/L. Uz iste uvjete odredili smo i granicu kvantifikacije trombocita, u nizu od (60, 10, 5, 3)x10^9/L, te dobili granicu od 5x10^9/L. Usporedbom dobivenih rezultata iz naših mjerenja i podataka dobivenih iz priručnika za hematološki brojač XN-1000, utvrdili smo kako su rezultati za leukocite podudarni s deklaracijom proizvođača. Dobiveni rezultat za trombocite bio je bolji od deklariranog od strane proizvođača (10x10^9/L) pri našim uvjetima prihvaćanja netočnosti i preciznosti. To je vrlo korisno u svakodnevnoj praksi, posebice u specijalističkim laboratorijima ustanova kojima gravitira velik broj onkoloških bolesnika. Less

Abstract (english)

Sysmex XN-1000 hematology analyzer is widely used and reliable analyzer in hematology laboratories. Goal of this research was to check the limit of quantification (LoQ) of leukocytes using „low white blood cell“ mode which is being used ever more frequently, especially for samples of immunosupressed pacients. Therefore, it is very important that laboratory personnel knows which given value can be validated as accurate and precise. Also, as part of our research, we have checked the limit... More of quantification of platelets in whole blood mode. To determine limit of quantification of leukocytes we used native samples which had extremely low leukocyte count (<0,2x10^9/L), as well as samples with higher white blood cell count (>1x10^9/L) dilluted with plasma, so it would be as close to matrix of a native sample. „Low-WBC“ mode uses so called WDF channel. It performs every measurement in triplicate which increases procedure accuracy. Dilution of samples for measurements of platelet count was made using plasma obtained by twicely repeated centrifuge at 4000 rpm. Firstly, we have measured the limit of blank (LoB), it turned out to be 0.00 x10^9/L. Leukocyte limit of quantification was determined experimentally, by continuouns measurements of ever lower leukocyte concentracions (1.0, 0.8, 0.5, 0.2, 0.1, 0.09,
0.07, 0.05, 0.03) x10^9/L. Furthermore, using the previously agreed criteria for highest acceptable impreccision and inaccuracy (bias and CV) of 10% we determined the leukocyte LoQ at 0.1x10^9/L. Using the same criteria the platelet LoQ was determined at 5x10^9/L after testing series of samples of (60, 10, 5, 3)x10^9/L. Comparing our test results and dana provided by Sysmex XN-1000 manual, we have concluded that the leukocyte LoQ results match the data provided by the producer. Result of platelet LoQ was even better than the one provided (at 10x10^9/L), using our criteria of accepting 10% bias and CV. To conclude, these findings are very useful in day-to-day practise, especially for the specialized laboratories of medical institutions that take care of a large number of oncological patients. Less

• Keywords: leukociti; trombociti; hematološki brojač; Sysmex XN-1000
• Keywords (english): leukocytes; thrombocytes; hematology analyzer; Sysmex XN-1000
• Language: croatian
• URN:NBN: urn:nbn:hr:163:905256
• Study programme: Title: Medical biochemistry; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra medicinske biokemije (magistar/magistra medicinske biokemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-04-08 06:55:27