Razvojne promjene N-glikana neuroglikoma korteksa mozga štakora

master's thesis

• Title (english): N-glycan Profile Changes in Rat Brain Neuroglycome During Brain Development
• Author: Matija Salopek
• Mentor: Gordan Lauc (mentor)
• Committee member: Gordan Lauc (predsjednik povjerenstva)
• Committee member: Ana-Marija Domijan (član povjerenstva)
• Committee member: Toma Keser (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of biochemistry and molecular biology); Zagreb
• Defense date and country: 2018-09-17, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Analiza N-glikana tkiva mozga postaje područje sve većeg interesa glikobiologa. Poznato je
da N-glikoprofil određenog tipa tkiva ovisi o interakcijama unutar kompleksne mreže čimbenika.
Jedan od važnih čimbenika je vrijeme, odnosno dob jedinke. U ovom radu, nakon izolacije,
pročišćavanja i fluorescentnog obilježavanja reducirajućeg kraja N-glikani korteksa mozga štakora iz 3
različite vremenske točke razvoja (24 i 48 sati postnatalne dobi, odrasla dob) analizirani su ranije
razvijenom normalnofaznom kromatografskom metodom uz fluorescencijsku detekciju (HILICUPLC-
FLR; Klarić i Gudelj, 2017) uz manje modifikacije postupka. Rezultati kromatografske analize
podijeljeni su u 48 frakcija, a kompozicije N-glikana u svakoj frakciji određene su interpretacijom
ESI(+)-MS/MS spektara. Rezultati pokazuju statistički značajne razlike odraslih i mladih jedinki u
frakcijama: 5 (odrasli – dominantna kompozicija: H3N4F1, mladi – dominantna kompozicija:
H3N5), 16 (odrasli: H4N4F2 i H5N4F1, mladi: H5N4F1, izostanak H4N4F2), 27 (27.1 - odrasli:
H7N2Phos2, H6N4F2, mladi: H7N2Phos2; 27.2 H5N5F1S1, H5N4F2S1), 34 (odrasli: H5N4F2S2,
H5N4F1S3, H5N4F1S2, H5N6F3, mladi: H5N4F1S2). Iz ovog istraživanja nije moguće utvrditi
točan utjecaj određene kompozicije/strukture, no nije moguće niti isključiti postojanje modulatorne
uloge pojedinih kompozicija prilikom razvoja korteksa mozga štakora.

Abstract (english)

Brain tissue N-glycans analysis is becoming a field of intensive glycobiological research. It
has been shown that the N-glycoprofile of a specific tissue types results from a complex network of
interactions of various factors, one of which is time – or to be precise – the age of an organism. In this
paper after isolation, purification and reducing end fluorescent labeling rat brain cortex N-glycans
from 3 different temporal points (24 and 48 h post natum, adult brains) were analysed using a HILICUPLC-
FLR method reported earlier (Klarić i Gudelj, 2017) with minor protocol modifications.
Chromatograms were divided into 48 distinct fractions and N-glycan compositions found in each
fraction were elucidated using ESI(+)-MS/MS spectra. Here, we report 4 major statistical differences
with possible biological implications in fractions: : 5 (adults–dominant composition(s): H3N4F1,
neonates–dominant composition(s): H3N5), 16 (adults: H4N4F2 i H5N4F1, neonates: H5N4F1,
absence of H4N4F2), 27 (27.1 - adults: H7N2Phos2, H6N4F2, neonates: H7N2Phos2; 27.2
H5N5F1S1, H5N4F2S1), 34 (adults: H5N4F2S2, H5N4F1S3, H5N4F1S2, H5N6F3, neonates:
H5N4F1S2). Furthermore, comprehensive research is needed to help elucidate the exact impact of
these structures on brain cortex development in rats and other organisms.

• Keywords: N-glikani; neuroglikom; HILIC-UPLC; ESI-MS/MS
• Keywords (english): N-glycans; neuroglycome; HILIC-UPLC; ESI-MS/MS
• Language: croatian
• URN:NBN: urn:nbn:hr:163:181727
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-10-01 10:56:08