In vitro ispitivanje transkornealne permeabilnosti melatonina iz lecitinsko-kitozanskih nanočestica

master's thesis

• Title (english): In vitro transcorneal permeability of melatonin-loaded lecithin-chiotsan nanoparticels
• Author: Martin Štefek
• Mentor: Jasmina Lovrić (mentor)
• Committee member: Jasmina Lovrić (predsjednik povjerenstva)
• Committee member: Anita Hafner (član povjerenstva)
• Committee member: Dubravka Vitali Čepo (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical technology); Zagreb
• Defense date and country: 2019-09-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Topikalna primjena lijekova na oko je preferirani put primjene lijekova pri liječenju bolesti oka. Pod pojmom konvencionalnih oftalmičkih lijekova podrazumijevamo otopine i suspenzije lijeka koje se primjenjuju na površinu oka u obliku kapi za oko. Takovi farmaceutski oblici lijeka relativno brzo se uklanjaju s površine oka te je potrebno njihovo učestalo doziranje za postizanje farmakološkog učinka. To ima negativan učinak na adherenciju pacijenta. Terapijski nanosustavi predstavljaju jedno od inovativnih rješenja koja mogu dovesti do povećanja bioraspoloživosti topikalno primijenjenog oftalmičkog lijeka.
Glaukom je bolest karakterizirana oštećenjem vidnog živca i jedan od vodećih uzroka sljepoće. Povišeni očni tlak smatra se jednim od glavnih čimbenika rizika u razvoju glaukoma. Melatonin se istaknuo kao jedan od potencijalnih lijekova za uspiješno liječenje povećanog očnog tlaka i spriječavanja razvoja glaukoma . U ovom radu pripravljene su lecitinsko-kitozanske nanočestice s uklopljenim melatoninom, zadovoljavajućih fizičko-kemijskih svojstava. Kationski polimer
kitozan osigurava pozitivni površinski naboj nanočestica čime se potencijalno omogućava njihova interakcija s vodenomucinskim slojem suznog filma te produljuje vrijeme zadržavanja oftalmičke formulacije na površini oka. Pri ispitivanim uvjetima, lecitinsko-kitozanske nanočestice karakterizira produljeno oslobađanje melatonina. Permebilnost melatonina određena je korištenjem in vitro modela epitela rožnice temeljenog na HCE-T staničnoj liniji. Brzina oslobađanja melatonina korelira sa brzinom permeacije melatonina preko in vitro model epitela rožnice.

Abstract (english)

Topical administration of ophthalmic drugs is the preferred route of administration of drugs in the treatment of ocular diseases. The term conventional ophthalmic drugs is referred to drug solutions and suspensions that are applied to the eye surface in the form of eye drops. Such pharmaceutical dosage forms are removed relatively quickly from the eye surface, and their frequent dosing is required to achieve a pharmacological effect. This has a negative effect on patient adherence. Drug delivery nanosystems represent one of the innovative solutions that can increase the bioavailability of a topically applied ophthalmic drug. Glaucoma is a disease characterized by damage to the optic nerve and one of the leading causes of blindness. Elevated eye pressure is considered one of the major risk factors for glaucoma development. Melatonin has emerged as one of the potential drugs for successfully treating increased eye pressure and preventing glaucoma
development. In this work, melatonin-loaded lecithin-chitosan nanoparticles were prepared with satisfactory physicochemical properties. Incorporation of chitosan in the system povides nanoparticles with positive charged surface. This enables potential interation of nanoparticles with the mucoaqueous layer of the tear film and increase of retention time of the ophthalmic formulation on eye surface. Nanoparticles were characterised by prolonged melatonin release. Melatonin permeability was investigated using in vitro HCE-T corneal epithelial model. A correlation between melatonin in vitro release from nanoparticles and permeation rate was observed.

• Keywords: kitozan; melatonin; lecitin; nanočestice; glaukom; permeabilnost
• Keywords (english): chitosan; melatonin; lecithin; nanoparticles; glaucoma; permeability
• Language: croatian
• URN:NBN: urn:nbn:hr:163:979804
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-02-14 14:36:34