Bioluminiscencijski reporterski geni i njihova primjena u razvoju lijekova

master's thesis

• Title (english): Bioluminescent reporter genes and their application in drug development
• Author: Tea Tekić
• Mentor: Gordana Maravić Vlahoviček (mentor)
• Committee member: Gordana Maravić Vlahoviček (predsjednik povjerenstva)
• Committee member: Sanja Dabelić (član povjerenstva)
• Committee member: Erim Bešić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of biochemistry and molecular biology); Zagreb
• Defense date and country: 2019-07-12, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Bioluminiscencijski reporterski geni kodiraju enzime luciferaze koji kataliziraju oksidaciju luciferina popraćenu
emisijom svjetlosti. Brojne prirodne i rekombinantne luciferaze različitih svojstava komercijalno su dostupne u testnim
kompletima za određenu namjenu. Luciferazni test se može izvesti kao jednostruki, dvostruki ili višestruki za praćenje
više staničnih događaja istovremeno kombiniranjem luciferaza koje emitiraju svjetlost različitih boja.
Bioluminiscencijski reporterski geni široko se koriste za istraživanje genske ekspresije, protein-protein interakcija,
signalnih puteva i drugih staničnih događaja. Zbog jednostavnosti izvođenja, neinvazivnosti, visoke osjetljivosti i
linearnosti ispitivanja te pogodnog omjera cijene i učinkovitosti bioluminiscencijske metode su jedne od
najprikladnijih za primjenu u in vitro i in vivo postupcima u razvoju lijekova. Glavni načini na koje se
bioluminiscencija iskorištava u razvoju lijekova su visokoprotočno pretraživanje opsežnih knjižnica kemijskih spojeva
u potrazi za novim spojevima kandidatima za lijek i bioluminiscentno biološko oslikavanje pretkliničkih životinjskih
modela za procjenu učinkovitosti lijekova.

Abstract (english)

Bioluminescent reporter genes encode luciferases - enzymes that catalyze the reaction of luciferin oxidation
accompanied by light emission. Numerous wild type and recombinant luciferases of different properties are
commercially available in test kits for a particular purpose. Bioluminescent assay can be performed as a single, double
or multiple luciferase assay for monitoring multiple cellular events simultaneously by combining luciferases emitting
light of a different colour. Bioluminescent reporter genes are widely used to study gene expression, protein-protein
interactions, cell signaling pathways, and other cellular events. Due to simplicity of performance, noninvasiveness,
high sensitivity and linearity of the assay and good cost-efficacy ratio bioluminescent methods are among the most
suitable for drug development in vitro and in vivo. The main ways in which bioluminescence is used in drug
development are high throughtput screening of extensive chemical libraries in search of a new drug candidate and
bioluminescent molecular imaging in preclinical animal models for the assessment of drug efficacy.

• Keywords: bioluminiscencija; luciferaza; reporterski geni; razvoj lijekova
• Keywords (english): bioluminescence; luciferase; reporter genes; drug development
• Language: croatian
• URN:NBN: urn:nbn:hr:163:556418
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-02-21 16:01:54