Kvalitativna i kvantitativna analiza ukupnih polifenola i trjeslovina vrste Arbutus unedo L. (Ericaceae), populacije Lošinj i Koločep

master's thesis

• Title (english): Qualitative and quantitative analysis of total polyphenols and tannins of Arbutus unedo L. (Ericaceae), populations Lošinj and Koločep
• Author: Antonio Boroš
• Mentor: Renata Jurišić Grubešić (mentor)
• Committee member: Renata Jurišić Grubešić (predsjednik povjerenstva)
• Committee member: Živka Juričić (član povjerenstva)
• Committee member: Željka Vanić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical analysis); Zagreb
• Defense date and country: 2019-09-16, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

U okviru ovoga diplomskog rada provedena je kvalitativna i kvantitativna analiza ukupnih polifenola i
trjeslovina iz listova i plodova vrste Arbutus unedo L. prikupljenih 2013. godine na području Malog Lošinja
i Koločepa. Prisutnost trjeslovina dokazana je u svim ispitanim uzorcima kemijskim reakcijama stvaranja
obojenih produkata i taloga. Kvantitativna analiza ukupnih polifenola (UP) i trjeslovina (T) provedena je
spektrofotometrijskom metodom s Folin-Ciocalteuovim fenolnim reagensom. Najveći sadržaj UP
zabilježen je u listovima obične planike s otoka Lošinja i iznosio je 1,36±0,01% (720 nm), odnosno
1,39±0,01% (752 nm), dok je najmanji UP sadržaj zabilježen u plodovima iste biljne populacije i iznosio je
0,27±0,01% (720 nm), odnosno 0,28±0,01% (752 nm). Najveći udio T u ekstraktima listova izmjeren je u
uzorku s Lošinja (0,73±0,01%), dok koncentraciju T u plodovima ispitanih populacija obične planike nije
bilo moguće odrediti primijenjenom spektrofotometrijskom metodom.

Abstract (english)

This diploma thesis elaborates qualitative and quantitive analysis of total polyphenols and tannins
extracted from leaves and fruits of the species Arbutus unedo L. collected during 2013 in the area of Mali
Lošinj and Koločep. Presence of tannins had been proven in all tested samples using chemical reactions
of precipitation and forming of colored products. The quantitive analysis of total polyphenols (TP) and
tannins (T) was performed by spectrophotometric method with usage of Folin-Ciocalteu's phenol reagent.
The highest content of TP was found in the leaves of strawberry tree from the island of Lošinj and it
amounted 1.36±0.01% (720 nm), i.e. 1.39±0.01% (752 nm), while the lowest TP content was found in
fruits of the same herbal population and it totaled 0.27±0.01% (720 nm), i.e. 0.28±0.01% (752 nm). The
highest concentration of T in the extracts of leaves was also found in the sample from Mali Lošinj
(0.73±0.01%), while the used spectrophotometric method wasn't succesfull in measuring T concentration
in the fruits of tested populations of strawberry tree.

• Keywords: Arbutus unedo L.; polifenoli; trjeslovine; kvalitativna analiza; kvantitativna analiza; UV-Vis spektrofotometrija
• Keywords (english): Arbutus unedo L.; polyphenols; tannins; qualitative analysis; quantitative analysis; UV-Vis spectrophotometry
• Language: croatian
• URN:NBN: urn:nbn:hr:163:016106
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-02-28 12:34:22