Mogućnost povećanja topljivosti deksametazona primjenom neionskih površinski aktivnih tvari

master's thesis

• Title (english): The possibility of increasing the solubility of dexamethasone using non-ionic surfactants
• Author: Ana Rogar
• Mentor: Ivan Pepić (mentor)
• Committee member: Ivan Pepić
• Committee member: Anita Hafner
• Committee member: Lovorka Vujić
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical technology); Zagreb
• Defense date and country: 2015-09-18, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Bioraspoloživost djelatnih tvari uvelike ovisi o topljivosti same djelatne tvari stoga povećanje topljivosti pridonosi bioraspoloživosti djelatne tvari. Jedna od mogućnosti povećanja topljivosti je primjena neionskih površinski aktivnih tvari. Ispitana je modelna teško topljiva tvar deksametazon u ternarnim sustavima deksametazona, neionskih solubilizatora Pluronica® F68 i Cremophora® EL, Krebs-Ringerovog pufera kao složene otopine elektrolita pri temperaturi od 34 ºC karakteristiĉne za oko kao mjesto apsorpcije djelatne tvari.Sadržaj otopljenog/solubiliziranog deksametazona u filtratima odreĊen je spektrofotometrijskom metodom pri  = 241 nm, a veliĉina i disperznost micela u filtratima odreĊena je fotonskom korelacijskom spektroskopijom (PCS). Rezultati pokazuju da neionske površinski aktivne tvari Pluronic® F68 i Cremophor® EL povećavaju topljivost deksametazona. Cremophor® EL šest puta više povećava topljivost od Pluronica® F68. Djelatne tvari steroidne strukture poput deksametazona teško su topljivi u vodi te uklapanje u micele graĊene od neionskih površinski aktivnih tvari u velikoj mjeri poboljšava njihovu topljivost. Nastale otopine su prozirne i stabilne na sterilizaciju te udovoljavaju propisima za oĉnu primjenu. Ovo istraživanje omogućava jasnu viziju solubilizacijskog potencijala neionskih površinski aktivnih tvari.

Abstract (english)

The bioavailability of active substances greatly depends on the solubility of the active substance. Therefore, increase of the solubility contributes bioavailability of the active substance. One of the possibilities to increase the solubility is use of nonionic surfactants. For model, sparingly soluble substance dexamethasone is tested, in ternary systems of dexamethasone, nonionic solubilizers of Pluronic® F68 and Cremophor® EL, Krebs-Ringer buffer as compound solution of electrolits at 34 ºC, which is typical for the eye as a place of the absorption of active substance. The content of the solubilizeddexamethasone in the filtrates is defined by spectrophotometric method at  = 241 nm, and the size and dispersion of the micelles in the filtrates is defined by photon correlation spectroscopy (PCS). The results show that the nonionic surfactants Pluronic® F68 and Cremophor® EL increase the solubility of dexamethasone. Cremophor® EL increases the solubility six times more than Pluronic® F68. Active substances with steroid structure such as dexamethasone are hardly soluble in water, and fitting of the micella built of nonionic surfactants largely improves their solubility. The resulting solutions are clear and stable to sterilization, and they satisfy the regulations for eye application. This research enables the clear vision of solubilzation potential of nonionic surfactants.

• Keywords: topljivost; deksametazon; Pluronic ® F68; Cremophor ® EL; neionske površinski aktivne tvari
• Keywords (english): solubility; dexamethasone; Pluronic® F68; Cremophor® EL; nonionic surfactants
• Language: croatian
• URN:NBN: urn:nbn:hr:163:023027
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2016-02-16 15:11:13