Utjecaj akarboze na katalitičke aktivnosti alanin aminotransferaze i aspartat aminotransferaze u jetri kontrolnih i dijabetičnih CBA miševa

Scientific paper - Preliminary note / Short communication

• Title (english): Effect of acarbose on alanine aminotransferase and aspartate aminotransferase activities in the liver of control and diabetic CBA mice
• Author: Roberta Petlevski
• Author: Mirko Hadžija
• Author: Jana Lukac Bajalo
• Author: Dubravka Juretic
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of medical biochemistry and haematology)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Medical Biochemistry

Abstract (croatian)

Svrha ovog rada bila je ispitati kratkotrajni učinak 0.1% (m/m) akarboze u suhoj hrani na katalitičku koncentraciju specifičnih jetrenih enzima: alanin aminotransferaze (ALT) i aspartat aminotransferaze (AST) u jetri kontrolnih i dijabetičnih CBA miševa. Dijabetes je bio izazvan i.v. injekcijom aloksan-monohidrata u dozi od 75 mg kg -1 tjelesne mase miša sedam dana prije početka ishrane s akarbozom. U pokusu su ispitane četiri skupine CBA miševa: kontrolna (C) (n = 6) i dijabetična (D) (n = 8) skupina bile su sedam dana na standardnoj ishrani, te kontrolna (C / A – 100) (n = 8) i dijabetična (D / A-100) (n = 8) skupina koje su hranjene 0.1 % akarbozom umiješanom u standardnu hranu. U skupini D katalitička koncentracija ALT-a bila je značajno snižena u usporedbi s kontrolnom skupinom C. Sličan pad katalitičke koncentracije ALT-a zabilježen je i u jetri kontrolnih i dijabetičnih miševa hranjenih suhom hranom u koju je bila umiješana akarboza (0.1%). U ispitanim skupinama nisu zabilježene promjene u specifičnoj i ukupnoj aktivnosti AST-a.

Abstract (english)

The purpose of this study was to examine the short-term effects of diet containing 0.1% (m/m) of acarbose in standard laboratory chow on specific liver enzyme activities: alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in control and diabetic CBA mice. Diabetes was induced by intravenous injection of alloxan monohydrate in a dose of 75 mg kg-1 mouse body mass seven days before the treatment with acarbose. There were four groups of CBA mice in the experiment: control (C) mice (n = 6) and diabetic (D) mice (n = 8) fed standard chow; control (C/A-100) mice (n = 8) and diabetic (D/A-100) mice (n = 8) fed standard chow containing 0.1% acarbose. Diabetes induced the decrease of the ALT catalytic activities to 69.6% of control value. A similar level of decreased ALT catalytic activity was detected in the liver of control and diabetic mice fed with chow containing 0.1% acarbose. No changes in the specific and total activities of AST in the liver of the experimental groups were observed.

• Keywords (croatian): akarboza; alanin aminotransferaza; aspartat aminotransferaza; diabetes
• Keywords (english): acarbose; alanine aminotransferase; aspartate aminotransferase; diabetes
• Language: croatian
• Language: english
• Publication type: Scientific paper - Preliminary note / Short communication
• Publication status: Published
• Peer review: Peer review - international
• Publication version: Published version
• Journal title: Acta Pharmaceutica
• Numbering: vol. 56, no. 1, pp. 87-93
• p-ISSN: 1330-0075
• e-ISSN: 1846-9558
• URN:NBN: urn:nbn:hr:163:655999
• Publication: 2006
• Document URL: https://hrcak.srce.hr/4566
• Type of resource: Text
• Access conditions: Open access
• Created on: 2020-04-08 14:41:47