Mikotoksinogenost kliničkih i okolišnih Aspergillus fumigatus i A. flavus sojeva

Scientific paper - Original scientific paper

• Title (english): Mycotoxigenicity of clinical and environmental Aspergillus fumigatus and A. flavus isolates
• Author: Ivan Kosalec
• Author: Stjepan Pepeljnjak
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of microbiology)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract (croatian)

Analizirana je mikotoksinogenost pedeset kliničkih sojeva vrste A.fumigatus i trideset sojeva vrste A. flavus izoliranih iz imunokompromitiranih ispitanika u hematološkom odjelu. Kao usporedba, izoliran je isti broj okolišnih sojeva iz zemlje, komposta i
zraka. Utvrđeno je da samo 9 sojeva (18%) vrste A. fumigatus tvori gliotoksin sa srednjom vrijednošću 2,22 mg mL–1 (u rasponu od 0,5 do 5 mg mL–1). Tvorba aflatoksina B1 utvrđena je u 7 sojeva (23%) (raspon od 0,024 do 1,2 mg L–1) i u jednog kliničkog izolata (3%) A.flavus vrste (0,12 mg L–1). U skupini okolišnih sojeva utvrđena je tvorba aflatoksina B1u 11 sojeva (37%) (u rasponu od 0,024 do 1,2 mg L–1) i u jednog soja tvorba aflatoksina G1(0,024 mg L–1). Bioautoantibiogramom na tankoslojnoj kromatografskoj ploči s bakterijskom vrstom Bacillus subtilis NCTC 8236 utvrđeno je da samo gliotoksinogeni sojevi pokazuju baktericidnu aktivnost oko Rf vrijednosti koja odgovara gliotoksinu. Profili sekundarnih metabolita kliničkih i okološnih sojeva A. funmigatus i A. flavus vrsta su bili homogeni, osim za tvorbu gliotoksina koji je utvrđen samo u skupini kliničkih izolata A. fumigatus sojeva (18%).

Abstract (english)

Clinical isolates of fifty strains of A. fumigatus and 30 strains of A. flavus from immmunocompromised patients from the hematological unit were analyzed for mycotoxin production and compared with the same number of environmental isolates (from soil, compost, and air). Only 9 (18%) strains of A. fumigatus produced gliotoxin in a mean concentration 2.22 mg mL-1 (range 0.5-5 mg mL-1). Aflatoxin B1 was detected in 7 (23%) isolates (range from 0.02 to 1.2 mg L-1) and aflatoxin G1 in one (3%) of clinical A. flavus isolates (0.12 mg L-1). In the group of environmental isolates, 11 (37%) were positive for aflatoxin B1 production (range from 0.02 to 1.2 mg L-1) and one for aflatoxin G1 (0.02 mg L-1). Bioautoantibiogram ("bioassay in situ") on TLC plates against Bacillus subtilis NCTC 8236 showed that only gliotoxin-producing strains have bactericidal activity of Rf values corresponding to gliotoxin. The secondary-metabolite profiles of clinical and environmental A. fumigatus and A. flavus isolates were homogenous, except for gliotoxin production, which was detected only in the group of clinical isolates of A. fumigatus (18%).

• Keywords (croatian): Aspergillus; A. fumigatus; A. flavus; gliotoksin; aflatoksin; mikotoksin; tvorba in vitro; sekundarni metaboliti; plijesni
• Keywords (english): Aspergillus; A. fumigatus; A. flavus; gliotoxin; aflatoxin; mycotoxin; in vitro production; secondary metabolites; moulds
• Language: croatian
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review - international
• Publication version: Published version
• Journal title: Acta Pharmaceutica
• Numbering: vol. 55, no. 4, pp. 365-375
• p-ISSN: 1330-0075
• e-ISSN: 1846-9558
• URN:NBN: urn:nbn:hr:163:241452
• Publication: 2005
• Document URL: https://hrcak.srce.hr/18120
• Type of resource: Text
• Access conditions: Open access
• Created on: 2020-04-08 15:54:40