Antimikotsko djelovanje tekućeg ekstrakta i eteričnog ulja iz plodova aniša (Pimpinella anisum L., Apiaceae)

Scientific paper - Original scientific paper

• Title (english): Antifungal activity of fluid extract and essential oil from anise fruits (Pimpinella anisum L., Apiaceae)
• Author: Ivan Kosalec
• Author: Stjepan Pepeljnjak
• Author: Danica Kuštrak
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy)
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of microbiology)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract (croatian)

Ispitana je antimikotsko djelovanje tekućeg ekstrakta i eteričnog ulja iz plodova aniša (Pimpinella anisum L., Apiaceae) na kliničke izolate sedam vrsta kvascima sličnih gljivica i četiri vrste dermatofita. Za ispitivanje antifungalne aktivnosti upotrebljene su metode difuzije cilindrima i metode razrjeđivanja u tekućem hranilištu. Tekući ekstrakt aniša pokazuje antifungalnu aktivnost na vrste Candida albicans, C. parapsilosis, C. tropicalis, C. pseudotropicalis i C. krusei s vrijednostima minimalne inhibitorne koncentracije (MIK) između 17 i 20% (V/V). Vrsta C. glabrata je rezistentna na djelovanje esktrakta, a uočeno je promotivno djelovanje ekstrakta na rast Geotrichum sp. Esktrakt aniša pokazuje inhibiciju rasta dermatofita (Trichophyton rubrum, T. mentagrophytes, Microsporum canis i M. gypseum) s MIK vrijednostima između 1,5 i 9,0% (V/V). Eterično ulje aniša pokazuje jaku antifungalnu aktivnost na kvascima slične gljivice s vrijednostima MIK-a nižima od 1,56% (V/V), a na dermatofite s vrijednostima MIK-a nižima od 0.78% (V/V). Utvrđena je značajna razlika (p < 0,01) između MIK-a tekućeg ekstrakta aniša i aniševog eteričnog ulja. Eterično ulje aniša pokazuje jaču antifungalnu aktivnost na kvascima slične gljivice i dermatofite s vrijednostima MIK-a između 0,10 i 1,56% (V/V).

Abstract (english)

Antifungal activities of fluid extract and essential oil obtained from anise fruits Pimpinella anisum L. (Apiaceae) were tested in vitro on clinical isolates of seven species of yeasts and four species of dermatophytes. Diffusion method with cylinders and the broth dilution method were used for antifungal activity testing. Anise fluid extract showed antimycotic activity against Candida albicans, C. parapsilosis, C. tropicalis, C. pseudotropicalis and C. krusei with MIC values between 17 and 20% (V/V). No activity was noticed against C. glabrata, and anis fruits extracts showed growth promotion activity on Geotrichum spp. Anise fruits extract inhibited the growth of dermatophyte species (Trichophyton rubrum, T. mentagrophytes, Microsporum canis and M. gypseum) with MIC values between 1.5 and 9% (V/V). Anise essential oil showed strong antifungal activity against yeasts with MIC lower than 1.56% (V/V) and dermatophytes with MIC lower than 0.78% (V/V). Significant differences in antifungal activities were found between anise fluid extract and anise essential oil (p < 0.01). Anise essential oil exhibited stronger antifungal activities against yeasts and dermatophytes with MIC values between 0.10 and 1.56% (V/V), respectively.

• Keywords (croatian): aniš; plod aniša; Pimpinella anisum (Apiaceae); tekući ekstrakt; eterično ulje; antifungalno djelovanje; Candida; dermatofit
• Keywords (english): anise; anise fruits; Pimpinella anisum (Apiaceae); fluid extract; essential oil; antifungal activity; Candida; dermatophyte
• Language: croatian
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review - international
• Publication version: Published version
• Journal title: Acta Pharmaceutica
• Numbering: vol. 55, no. 4, pp. 377-385
• p-ISSN: 1330-0075
• e-ISSN: 1846-9558
• URN:NBN: urn:nbn:hr:163:845664
• Publication: 2005
• Document URL: https://hrcak.srce.hr/19043
• Type of resource: Text
• Access conditions: Open access
• Created on: 2020-04-08 15:59:13