Antidepresivni učinak Hipericum perforatum L. mjeren pomoću dvije eksperimentalne metode na miševima

Scientific paper - Preliminary note / Short communication

• Title (english): The antidepressant activity of Hypericum perforatum L. measured by two experimental methods on mice
• Author: Lidija Bach-Rojecky
• Author: Zdenka Kalođera
• Author: Ita Samaržija
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy)
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacology)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract (croatian)

Farmakološki pristup liječenju depresije uključuje dugotrajnu prijmjenu antidepresiva, pri čemu se oni mogu primjenjivati kao monoterapija ili pak kao kombinacija više lijekova s različitim mehanizmima djelovanje. Hipericum perforatum L. (gospina trava) je jedini prirodni antidepresiv koji je djelotvoran u liječenju depresivnih poremećaja blagog i srednje jakog intenziteta. Za antidepresivno djelovanje gospine trave čini se da su najzaslužniji hipericin i hiperforin.
U ovom radu ispitivan je antidepresivni učinak niskih doza ekstrakta H. perforatum i to: 7, 35 i 70 mg kg-1 na miševima pomoću metode «prinudnog plivanja» i «vješanja za rep». Kao pokazatelj antidepresivnog djelovanja mjereno je vrijeme mirovanja životinja- što je ono bilo kraće, aktivnost životinja je bila veća, pa je antidepresivni učinak bio jači. Povećanjem doze sa 7 mg kg-1 na 35 odnosno 70 mg kg-1, antidepresivni učinak ekstrakta H. perforatum bio je veći u odnosu na kontrolnu skupinu životinja i to za 10,1%, 25,8% i 38,6% u metodi plivanja, odnosno za 12,7%, 16,5% i 24,5% u metodi vješanja za rep. Rezultati ovog rada ukazuju na antidepresivno djelovanje ekstrakta gospine trave već nakon jednokratne primjene niskih doza u oba korištena eksperimentalna modela.

Abstract (english)

The pharmacological approach to the treatment of depression includes a long-term employment of antidepressants, either in the form of monotherapy or as a combination of several antidepressants with various mechanisms of action. Hypericum perforatum L. (St. John's wort) is the only natural antidepressant. Several constituents of the extract, such as hypericin and hyperforin seem to be important for this effect. H. perforatum is considered to be an effective alternative to other therapeutic agents in the treatment of mild to moderate depression.
The paper describes the investigation of the antidepressant effect of H. perforatum (doses 7, 35 and 70 mg kg-1 b.m.) on mice using the forced-swimming and tail suspension methods. As an indicator of the antidepressant effect, it was shown that the immobility time of animals in the forced-swimming and tail-suspension experiments was shorter, i.e. the activity of the animals was higher. With single doses of extract suspension increasing from 7, over 35 to 70 mg kg-1 of extract suspension, the antidepressant effect increased in proportion by 10.1%, 25.8% and 38.6% in the swimming method, and by 12.7%, 16.5% and 24.5% in the tai-suspension method compared to controls. H. perforatum extract displays a dose-dependent antidepressant effect at a dose as low as 7 mg kg-1. Both models have proved to be equally valuable for demonstration of substances with a potential antidepressant effect.

• Keywords (croatian): Hypericum perforatum, L. Hypericaceae; antidepresivni učinak; životinjski modeli depresije
• Keywords (english): Hypericum perforatum, L. Hypericaceae; antidepressant effect; forced swimming test; tail suspension test
• Language: croatian
• Language: english
• Publication type: Scientific paper - Preliminary note / Short communication
• Publication status: Published
• Peer review: Peer review - international
• Publication version: Published version
• Journal title: Acta Pharmaceutica
• Numbering: vol. 54, no. 2, pp. 157-162
• p-ISSN: 1330-0075
• e-ISSN: 1846-9558
• URN:NBN: urn:nbn:hr:163:068807
• Publication: 2004
• Document URL: https://hrcak.srce.hr/16828
• Type of resource: Text
• Access conditions: Open access
• Created on: 2020-04-09 13:44:56