Antimikrobni učinak etanolnog ekstrakta sjemenki i pulpe ploda grejpa

Scientific paper - Preliminary note / Short communication

• Title (english): Antimicrobial activity of grapefruit seed and pulp ethanolic extract
• Author: Zdenka Cvetnić
• Author: Sanda Vladimir-Knežević
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy)
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of microbiology)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract (croatian)

Ispitano je djelovanje etanolnog ekstrakta sjemenki i pulpe ploda grejpa (Citrus paradisi Macf., Rutaceae) na 20 sojeva bakterija i 10 sojeva kvasnica. Antibakterijski i antifugalni učinak ekstrakta testiran je postupkom difuzije na hranjivom agaru i standardnom metodom dilucije. Spektrofotometrijski je određeno da suhi ekstrakt sadrži 3,92% ukupnih polifenola, dok je udio flavonoida iznosio 0,11%. Prisutnost flavonona naringina i hesperidina u etanolnom ekstraktu potvrđena je tankoslojnom kromatografijom. Ekstrakt je pokazao najsnažniji učinak na vrstu Salmonella enteritidis (MIC 2,06%, m/V), dok je na ostale ispitane bakterije i kvasnice djelovao u koncentracijama od 4,13% do 16,50% (m/V).

Abstract (english)

Antibacterial and antifungal activity of ethanolic extract of grapefruit (Citrus paradisi Macf., Rutaceae) seed and pulp was examined against 20 bacterial and 10 yeast strains. The level of antimicrobial effects was established using an in vitro agar assay and standard broth dilution susceptibility test. The contents of 3.92% of total polyphenols and 0.11% of flavonoids were determined spectrometrically in crude ethanolic extract. The presence of flavanones naringin and hesperidin in the extract was confirmed by TLC analysis. Ethanolic extract exibited the strongest antimicrobial effect against Salmonella enteritidis MIC 2.06%, m/V). Other tested bacteria and yeasts were sensitive to extract concentrations ranging from 4.13% to 16.50% (m/V).

• Keywords (croatian): Citrus paradisi (Rutaceae); plod grejpa; ekstrakt sjemenki i pulpe; antimikrobni učinak
• Keywords (english): Citrus paradisi (Rutaceae); grapefruit seed and pulp extract; antimicrobial activity
• Language: croatian
• Language: english
• Publication type: Scientific paper - Preliminary note / Short communication
• Publication status: Published
• Peer review: Peer review - international
• Publication version: Published version
• Journal title: Acta Pharmaceutica
• Numbering: vol. 54, no. 3, pp. 243-250
• p-ISSN: 1330-0075
• e-ISSN: 1846-9558
• URN:NBN: urn:nbn:hr:163:819781
• Publication: 2004
• Document URL: https://hrcak.srce.hr/16867
• Type of resource: Text
• Access conditions: Open access
• Created on: 2020-04-09 14:08:40