Koncentracija malondialdehida u NOD miševa tretiranih akarbozom

Scientific paper - Original scientific paper

• Title (english): Concentration of malondialdehyde in a NOD mice treated with acarbose
• Author: Roberta Petlevski
• Author: Dubravka Juretić
• Author: Mirko Hadžija
• Author: Milivoj Slijepčević
• Author: Jana Lukač-Bajalo
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of medical biochemistry and haematology)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Medical Biochemistry

Abstract (croatian)

Cilj: Malondialdehid (MDA) je jedan od toksičnih produkata lipidne peroksidacije, a koristi se u evaluaciji oksidativnog stresa tijekom šećerne bolesti. Cilj ovog rada bio je ispitati učinak akarboze (inhibitora α-glukozidaza) na koncentraciju glukoze u serumu i MDA u homogenatu jetre NOD (engl. non-obese diabetic) miševa.
Materijali i metode: U NOD miševa šećerna bolest inducirana je i.v. aplikacijom aloksan-monohidrata (75 mg/kg t. mase). NOD miševi podijeljeni su u 4 skupine (n=6): Kontrolni, zdravi NOD miševi (K), Kontrolni, zdravi NOD miševi tretirani 7 dana akarbozom (K/A), dijabetični NOD miševi (D) te dijabetični NOD miševi tretirani 7 dana akarbozom (D/A).
Koncentracija glukoze u krvi izmjerena je glukoza oksidaza-peroksidaza metodom, a koncentracija MDA u homogenatu jetre određena je upotrebom metode s tiobarbiturnom kiselinom.
Rezultati: Nakon sedmodnevnog tretmana akarbozom zabilježeno je statistički značajno smanjenje koncentracije glukoze u krvi u skupini D/A u odnosu na skupinu D (p<0.05), a isto tako je uočen i statistički značajan pad koncentracije MDA (p<0.05).
Zaključak: Ovi rezultati potvrđuju pozitivan antioksidacijski učinak akarboze što se može objasniti njezinim antihiperglikemijskim djelovanjem.

Abstract (english)

Aim. Malondialdehyde (MDA) is one of the toxic product of the lipid peroxida-tion, and that plays an important role in the evaluation of oxidative stress in the diabetes mellitus. The aim of this study was to study the effect of acarbo-se (α-glucosidase inhibitor) on serum glucose concentration and MDA in liver homogenate of NOD (non-obese diabetic) mice.
Material and methods. Diabetes was induced in mice by i.v. administration of alloxan-monohydrate (75 mg/kg b.w.). The mice were divided into 4 groups (N=6): control, healthy mice (C), control healthy mice on 7-day treatment with acarbose (C/A), NOD mice (D), and NOD mice on 7-day acarbose treatment (D/A).
The levels of blood glucose and of MDA in liver homogenate were determined by glucose oxidase-peroxidase method and a method with thiobarbituric acid, respectively.
Results. Significant reduction in blood glucose levels was observed after 7-day administration of acarbose in D/A group compared to D group (p<0.05), together with a significant fall in MDA concentration (p<0.05).
Conclusion. This results confirmed thefavorable antioxidative effect of acarbose which may be explained by its antihyperglycemic action.

• Keywords (croatian): oksidativni stres; šećerna bolest; lipidna peroksidacija; akarboza
• Keywords (english): oxidative stress; diabetes; lipid peroxidation; acarbose
• Language: croatian
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review - international
• Publication version: Published version
• Journal title: Biochemia Medica
• Numbering: vol. 16, no. 1, pp. 43-49
• p-ISSN: 1330-0962
• e-ISSN: 1846-7482
• URN:NBN: urn:nbn:hr:163:066225
• Publication: 2006
• Document URL: https://hrcak.srce.hr/11534
• Type of resource: Text
• Access conditions: Open access
• Created on: 2020-04-10 15:36:20