Spectrophotometric determination of malondialdehyde in urine suitable for epidemiological studies

Scientific paper - Original scientific paper

• Author: Tin Weitner
• Author: Suzana Inić
• Author: Jasna Jablan
• Author: Mario Gabričević
• Author: Ana-Marija Domijan https://orcid.org/0000-0001-5645-9732
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of analytical chemistry)
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of general and inorganic chemistry)
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry; Analytic Chemistry

Abstract (english)

A reliable method for spectrophotometric determination of urinary malondialdehyde (MDA), according to the thiobarbituric acid (TBA) assay, is described. To account for matrix interference and differences in individual urine composition, standard addition procedure was applied. The method is adequately selective (LoQ = 0.09 μM in the presence of 0.1 M creatinine and 0.5 M urea) and reliable (within-day and between-day variability of less than 5 %). The mean level of urinary MDA was 1.52 ± 0.73 µM that is in good agreement with spectrofluorometric determination (1.20 ± 0.56 μM; p = 0.085) as well as with previous studies that used HPLC. Furthermore, it is demonstrated that MDA is stabile in urine at room temperature for 24 h and when stored at –20 °C for 6 months. The described method enables simple, rapid and cost-effective determination of urinary MDA as a relevant and non-invasive marker of “whole-body” oxidative stress.
<br><a rel="license" href="http://creativecommons.org/licenses/by/4.0/"><img alt="Creative Commons License" style="border-width:0" src="https://i.creativecommons.org/l/by/4.0/80x15.png" /></a> This work is licensed under a <a rel="license" href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution 4.0 International License</a>.

• Keywords (english): malondialdehyde; thiobarbituric acid assay; urine; interferences; stability
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review - international
• Publication version: Published version
• Journal title: Croatica Chemica Acta
• Numbering: vol. 89, no. 1, pp. 133-139
• p-ISSN: 0011-1643
• e-ISSN: 1334-417X
• DOI: https://doi.org/10.5562/cca2902
• URN:NBN: urn:nbn:hr:163:584413
• Publication: 2016
• Document URL: https://hrcak.srce.hr/160091
• Type of resource: Text
• Access conditions: Open access
• Created on: 2020-04-14 17:05:26