Catalytic intermediates in the reaction of deuteroferriheme with m-chloroperoxybenzoic acid

Scientific paper - Original scientific paper

• Author: Vera Imper
• Author: Harald E. Van Wart
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of physical chemistry)
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract (english)

Deuteroferriheme (DFH) reacts with m-chloroperoxybenzoic acid
(m-CPBA) to produce areaction intermediate whose optical spectrum
resembles that of horseradish peroxidase (HRP) compound o. To exclude problems related to aggregation, the fraction of dimers
was maintained below 2%. Under these conditions, the reaction of
DFH with m-CPB in 10 mM phosphate containing 1% u/u 1,2-dimethoxyethane (I = O.lM NaCI) at pH 5.5 at 1 °C was shown to
produce an intermediate that has a distinctive Soret band near 350
nm, in agreement with earlier observations in the literature. The
EPR spectrum of this intermediate indicates that it contains half ferric iron and cannot be a ferryl dimer. Its structure is more consistent with that of a ferric, ferryl dimer and a scheme supporting its formation has been proposed.

• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review - international
• Publication version: Published version
• Journal title: Croatica Chemica Acta
• Numbering: vol. 69, no. 3, pp. 997-1006
• p-ISSN: 0011-1643
• e-ISSN: 1334-417X
• URN:NBN: urn:nbn:hr:163:237744
• Publication: 1996
• Document URL: https://hrcak.srce.hr/177127
• Type of resource: Text
• Access conditions: Open access
• Created on: 2020-04-29 14:26:13