master's thesis
Optimization of extraction conditions for the analyses of dietary supplements used by women in menopause

Helena Turkalj (2015)
Sveučilište u Zagrebu
Farmaceutsko-biokemijski fakultet
Zavod za analitiku i kontrolu lijekova
Metadata
TitleOptimizacija ekstrakcijskih uvjeta za analizu proizvoda namjenjenih ženama u menopauzi
AuthorHelena Turkalj
Mentor(s)Miranda Sertić (thesis advisor)
Abstract
Menopauza je vrijeme u životu žene kada njezine mjesečnice prestaju i tijelo prolazi kroz mnoge promjene praćene brojnim neugodnim simptomima. Danas žene ulaze u menopauzu ranije nego nekoliko desetljeća ranije. Kod mnogih žena simptomi menopauze su toliko jaki da ometaju svakodnevne aktivnosti. Zbog toga mnoge žene koriste dodatke prehrani za ublažavanje tih neugodnih simptoma. Sve se više dodataka prehrani pojavljuje na tržištu te stoga raste potreba za kontrolom kvalitete tih proizvoda. Velika je važnost razvoja novih, selektivnih, osjetljivih i točnih analitičkih metoda za analizu aktivnih sastojaka u takvim proizvodima. Cilj ovoga rada bio je optimizirati ekstrakcijske uvjete za analizu fitoestrogena i njihovo kvantitativno određivanje u različitim biljnim materijalima i dodatcima prehrani namijenjenima ženama u menopauzi. Za optimizaciju ekstrakcijskih uvjeta korištena su tri biljna materijala (soja, konopljika i hmelj). Biljni materijal je homogeniziran, a aktivni sastojci su ekstrahirani u etanolu ili acetonitrilu. Otapala su korištena u koncentracijama 100%, 75% i 50%. Također su istražena različita vremena ekstrakcije (15 min, 30 min i 60 min). Ekstrakcija je provedena pri sobnoj temperaturi korištenjem ultrazvučne kupke. Nakon analize uzoraka novom razvijenom i validiranom LC/DAD/MS/MS metodom, korištenjem Agilent 1100 Series LC/MSD Trap System, kao najbolje otapalo za izolaciju flavonoida iz hmelja je odabran 100% etanol pri ekstrakciji u trajanju 15 minuta.
Keywordsextraction procedure menopause flavonoids liquid chromatography mass spectrometry
Parallel title (English)Optimization of extraction conditions for the analyses of dietary supplements used by women in menopause
Committee MembersMiranda Sertić
Biljana Nigović
Sandra Šupraha Goreta
GranterSveučilište u Zagrebu
Farmaceutsko-biokemijski fakultet
Lower level organizational unitsZavod za analitiku i kontrolu lijekova
PlaceZagreb
StateCroatia
Scientific field, discipline, subdisciplineBIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Study programme typeuniversity
Study levelintegrated undergraduate and graduate
Study programmePharmacy
Academic title abbreviationmag. pharm.
Genremaster's thesis
Language Croatian
Defense date2015-09-25
Parallel abstract (English)
Menopause is a time in a women`s life when her periods eventually stop and the body goes through many changes accompanied by numerous unpleasant menopausal symptoms. Today, women enter the menopause earlier than couple of decades ago. In many women, symptoms of menopause are so strong that they interfere with their daily life activities. For that reason, many women use dietary supplements for the relief of these uncomfortable symptoms. More and more dietary supplements for menopausal symptoms appear on the market and therefore there is a growing need for quality assessment of these products. Development of new, selective, sensitive and accurate analytical methods for the analysis of active compounds in such products is very important. The aim of this study was to optimize extraction conditions for the analysis of active phytoestrogenic ingridients and their quantitative determination in various herbal material and dietary supplements. For the optimization of extraction conditions herbal material of 3 species (Glycine max L., Vitex agnus castus L., Humulus lupulus L.) was used. Material was homogenized and the active ingredients were extracted by ethanol and acetonitrile. Extraction solvents used were of 100%, 75% and 50% concentractions. Also, different extraction time, 15 min, 30 min and 60 min, was investigated. Extraction was performed at room temperature using an ultrasonic bath. After the analysis of the samples by newly developed and validated LC/DAD/MS/MS method, using Agilent 1100 Series LC/MSD trap system, best extraction solvent for flavonoids in Humulus lupulus proved to be 100% ethanol at 15 minutes extraction time.
Parallel keywords (Croatian)ekstrakcija menopauza flavonoidi tekućinska kromatografija masena spektrometrija
Resource typetext
Access conditionAccess restricted to students and staff of home institution
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:163:389462
CommitterPetra Gašparac