Primjena bioluminiscencije u istraživanju i razvoju lijekova

Professional paper - Professional paper

• Author: Tea Tekić
• Author: Gordana Maravić Vlahoviček
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of biochemistry and molecular biology)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Medical Biochemistry

Abstract (english)

Bioluminescence involves chemical reactions accompanied by
light emission that take place in living organisms. A bioluminescent
reaction takes place between the luciferin molecule and the luciferase
enzyme that catalyzes its oxidation. Numerous luciferases and luciferins of different
properties have been isolated from various terrestrial and marine organisms,
and recently several recombinant luciferases and synthetic luciferin with
enhanced features have been created. Methods that utilize the bioluminescent
reaction of luciferase and luciferins are easy to perform and 11011-invasive, providing
high sensitivity and linearity of the assay. Therefore, the application of bioluminescence
is appropriate for specific stages of drug development. The use of
bioluminescence offers numerous advantages in discovering new drug candidate
compounds if used for high-throughput screening of extensive chemical
compound libraries and in the preclinical phase of drug efficacy assessment in
bioluminescent animal models.

• Keywords (croatian): bioluminiscencija; razvoj lijekova; luciferin; luciferaza
• Language: croatian
• Publication type: Professional paper - Professional paper
• Publication status: Published
• Peer review: Peer review - domestic
• Publication version: Published version
• Journal title: Farmaceutski glasnik
• Numbering: vol. 76, no. 3, pp. 185-196
• p-ISSN: 0014-8202
• URN:NBN: urn:nbn:hr:163:863195
• Publication: 2020
• Type of resource: Text
• Access conditions: Open access
• Created on: 2020-06-05 10:49:42