Liposomi s kalceinom: odabir optimalne metode priprave

Scientific paper - Original scientific paper

• Author: Nina Mandić
• Author: Željka Vanić
• Author: Jelena Filipović-Grčić
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical technology)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract (english)

The aim of our study was to develop a liposomal drug
carrier system able to provide appropriate trapping efficiency of hydrophilic
substance. Since majority of hydrophilic drugs are of low molecular weight,
calcein was chosen as a model compound. To optimize the preparation of
liposomes with regard to size and entrapment efficiency, liposomes containing
calcein were prepared by different methods: conventional and modified
film methods as well as proliposome method. Additionally, a dehydration-rehydration
procedure was applied on the liposomes prepared by the both
film methods. Ali preparations were extruded through polycarbonate membrane
filters to achieve liposomes with homogenous size distribution. Regardless
of the preparation method, all extruded liposomes were of mean diameter
between 240 and 280 nm. However, encapsulation of calcein into liposomes
differed far various preparation methods. Liposomes prepared by the
modified film method could entrap more of the hydrophilic marker than
those prepared by the conventional hydration method. Although the dehydration-
rehydration procedure could slightly enhance the trapping of calcein
into liposomes prepared by conventional film method, the extremely high
encapsulation of calcein was achieved into proliposomes (> 40 %). Therefore,
the proliposome method would be the right choice of preparation procedure
far entrapment of hydrophilic substances.

• Keywords (croatian): liposomi; kalcein
• Language: croatian
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review - domestic
• Publication version: Published version
• Journal title: Farmaceutski glasnik
• Numbering: vol. 68, no. 11, pp. 697-708
• p-ISSN: 0014-8202
• URN:NBN: urn:nbn:hr:163:930783
• Publication: 2012
• Type of resource: Text
• Access conditions: Open access
• Created on: 2020-09-29 11:58:07