Ciklodekstrinsko-polianhidridne nanočestice s melatoninom

master's thesis

• Title (english): Melatonin loaded cyclodextrin-poly(anhydride) nanoparticles
• Author: Hrvoje Jurinić
• Mentor: Mario Jug (mentor)
• Committee member: Mario Jug (predsjednik povjerenstva)
• Committee member: Ivan Pepić (član povjerenstva)
• Committee member: Dubravka Vitali Čepo (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical technology); Zagreb
• Defense date and country: 2018-05-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Svrha ovoga rada bilo je ispitivanje utjecaja HP-β-ciklodekstrina na svojstva pripravljenih nanočestica i uklapanje melatonina u njih. Pripravljene su ne-PEGilirane (mukoadhezivne) i PEGilirane (mukopenetrativne) nanočestice sintetskog kopolimera metil-vinil-etera i anhidrida maleinske kiseline (GantrezTM AN-169) metodom nanoprecipitacije. Melatonin je uklopljen u nanočestice u obliku krutog liofiliziranog kompleksa s HP-β-ciklodekstrinom, in situ pripremljene vodene otopine kompleksa te in situ stvorenog kompleksa pri formiranju nanočestica HP-β-ciklodekstrinom modificiranog kopolimera. Dobivene su nanočestice podvrgnute procesu sušenja smrzavanjem kako bi im se povećala stabilnost, nakon čega su rekonstituirane i analizirane. Rezultati pokazuju da su dobivene nanočestice prikladne veličine za primjenu na sluznicu te da su stabilne i monodisperzne, a liofilizacijom dolazi do porasta veličine, smanjenja indeksa polidisperznosti te u slučaju mukoadhezivinh čestica smanjenja zeta potencijala. Uklapanje melatonina je relativno nisko te se pokazalo da ciklodekstrini, suprotno očekivanjima, smanjuju uklapanje melatonina u nanočestice. Ipak, sadržaj melatonina je dostatan za postizanje pozitivnih terapijskih učinaka u lokalnom liječenju bolesti parodonta.

Abstract (english)

The aim of this work was to study the effect of HP-β-cyclodextrin on physical and chemical properties of
nanoparticles and encapsulation of melatonin. Using nanoprecipitation method non-PEGylated
(mucoadhesive) and PEGylated (mucopenetrative) nanoparticles were formed from the copolymer of
methyl vinyl ether and maleic anhydride (GantrezTM AN-169). Different types of inclusion complexes were
used to encapsulate melatonin such as a solid inclusion complex between melatonin and HP-β-cyclodextrin,
in situ prepared aqueous solution of the complex and complex formed in situ during the formation of
nanoparticles from HP-β-cyclodextrin-modified copolymer. In order to increase stability of nanoparticles,
they were subjected to freeze-drying process. The resulting nanoparticles displayed an adequate size for
mucosal drug delivery and were stabile and monodisperse. Freeze-drying caused an increase in size,
reduction of PDi and a reduction of zeta potential of mucoadhesive nanoparticles. Cyclodextrins caused a
reduction of the encapsulation efficiency of melatonin into nanoparticles. In spite of relatively low contents
of melatonin, it is still sufficient to obtain positive therapeutic effects for local treatment of periodontal
diseases.

• Keywords: nanočestice; sluznica; mucini; melatonin; ciklodekstrini; polietilenglikol
• Keywords (english): nanoparticles; mucus; mucins; melatonin; cyclodextrins; polyethylene glycol; drug loading
• Language: croatian
• URN:NBN: urn:nbn:hr:163:131376
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-04-19 11:11:09