Optimizacija metode plamene atomske apsorpcijske spektrofotometrije u svrhu određivanja kalcija u uzorcima žitarica

master's thesis

• Title (english): Optimisation of flame atomic absorption spectroscopy method due to calcium quantification in cereal samples
• Author: Teo Vučković
• Mentor: Jasna Jablan (mentor)
• Committee member: Jasna Jablan (predsjednik povjerenstva)
• Committee member: Mila Lovrić (član povjerenstva)
• Committee member: Maja Bival Štefan (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of analytical chemistry); Zagreb
• Defense date and country: 2020-05-08, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Kalcij je najzastupljeniji mineral u čovjekovom organizmu. Uloga kalcija u ljudskom
organizmu je od velike važnosti za normalan metabolizam. Važna je sastavnica kostiju i
zubiju, regulira kontrakciju i relaksaciju mišića, funkciju živčanog tkiva, zgrušavanje krvi,
utječe na regulaciju krvnog tlaka i provođenje prijenosnih signala živčanog sustava. Eliminira
se urinom i fecesom. Ukoliko unos kalcija i apsorpcija iz crijeva ne zadovoljavaju fiziološke
potrebe ljudskog organizma, tada se kalcij resorbira iz kostiju, što narušava zdravlje kostiju.
Kroničan deficit kalcija dovodi do smanjenja koštane mase, osteoporoze i učestale frakture
kostiju. Deficit kalcija moguće je spriječiti korištenjem namirnica obogaćenih kalcijem u
svakodnevnoj prehrani. Cilj ovoga rada bila je optimizacija metode plamene atomske
apsorpcijske spektrofotometrije u svrhu određivanja kalcija u uzorcima žitarica. Ispitivanja su
provedena na atomskom apsorpcijskom spektrofotometru Analyst 800 s deuterijskim
korektorom nespecifične površine na valnoj duljini od 422,7 nm. Postojeća metoda je
validirana ispitivanjem parametara validacije prema važećim smjernicama. Dobiveni rezultati
su pokazali da su izmjerene mase kalcija u uzorcima usporedive s deklariranim masama
proizvođača. Metoda se pokazala brzom, jeftinom, pouzdanom i pogodnom za određivanje
kalcija u različitim uzorcima žitarica.

Abstract (english)

Calcium is one of the most prevalent elements in the human body. As a such, it plays an
important role in normal human metabolism. It is a significant part of human bones and teeth,
regulates muscle contraction and relaxation, nerve tissue function, blood coagulation, affects
high blood pressure regulation, and conducting nerve system transmission signals. It is
eliminated by urine and feces. If calcium supply and bowel absorption are not sufficient to
satisfy the physiological needs of the human body, then calcium is resorbed from bones, which
impairs bones’ health. Chronic calcium deficit leads to bone mass decreasing, osteoporosis
and frequent bone fractures. Calcium deficit can be stopped using calcium enriched food daily.
The main purpose of this thesis was optimisation of flame atomic absorption spectroscopy due
to calcium quantification in cereal samples. Tests were done using atomic absorption
spectrophotometer Analyst 800 with non-specific surface deuterium corrector at the
wavelength of 422,7 nm. The existing method has been validated by the validation parameter
examination. Results show that measured calcium masses in samples are comparable with
declared masses. The method proved to be fast, cheap, reliable and appropriate for calcium
determination in different cereal samples.

• Keywords: atomska apsorpcijska spektrofotometrija; kalcij; žitarice
• Keywords (english): atomic absorption spectroscopy; calcium; cereals
• Language: croatian
• URN:NBN: urn:nbn:hr:163:728667
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-04-23 13:03:45