Utjecaj glikozilacije alfa1-kiselog glikoproteina na vezanje dipiridamola, imatiniba i azitromicina

master's thesis

• Title (english): Influence of glycosylation of alpha1-acid glycoprotein on the binding of dipyridamole, imatinib and azithromycin
• Author: Dunja Odobašić
• Mentor: Mario Gabričević (mentor)
• Committee member: Mario Gabričević (predsjednik povjerenstva)
• Committee member: Tin Weitner (član povjerenstva)
• Committee member: Sanja Dabelić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of general and inorganic chemistry); Zagreb
• Defense date and country: 2020-07-16, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

U sklopu ovoga diplomskoga rada provedeno je ispitivanje kako promjena glikozilacijskog uzorka α1-kiselog glikoproteina utječe na konstantu vezanja dipiridamola, imatiniba i azitromicina. Tehnika korištena u ispitivanju bila je fluorescencijska spektroskopija. Rezultati ispitivanja ukazuju na to da desijalinizacija α1-kiselog glikoproteina uzrokuje promjene u konstantama vezanja izabranih lijekova i α1-kiselog glikoproteina. Desijalinizacija navedenog proteina kod imatiniba uzrokuje blago povećanje konstante vezanja za razliku od dipiridamola gdje je učinak suprotan, odnosno nakon desijalinizacije α1-kiselog glikoproteina dipiridamol se slabije veže za takav oblik te je manja i konstanta vezanja. Zbog male konstante vezanje i premalog raspona koncentracija, mjerenja provedena za azitromicin nisu relevantna.

Abstract (english)

Within the scope of this diploma thesis, the effect of the binding constant of dipyridamole, imatinib and azithromycin on the α1-acid glycoprotein due to a change in the pattern of it's glycosylation was examined by fluorescence spectroscopy. Result indicates effect of desialylation of α1-acid glycoprotein on binding constants of selected drugs. Desialylation of α1-acid glycoprotein will slightly increase the binding constant for imatinib. On the other hand, desialylation of the same protein will reduce the binding constant for dipyridamole. Due to small binding constant and narrow range of used concentrations, measurements of azithromycin binding was not succesfull.

• Keywords: α1-kiseli glikoprotein; glikozilacija; desijalinizacija; konstanta vezanja; imatinib; dipiridamol; azitromicin
• Keywords (english): α1-acid glycoprotein; glycoylation; desialylation; bindindg constant; imatinib; dipyridamole; azithromycin
• Language: croatian
• URN:NBN: urn:nbn:hr:163:121774
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-04-23 13:47:00