Priprava i karakterizacija termoosjetljivog hidrogela s ekstraktom vrste Centella asiatica L. Urban

professional thesis

• Author: Gordana Naletilić
• Mentor: Jelena Filipović-Grčić (mentor)
• Committee member: Sanda Vladimir-Knežević (predsjednik povjerenstva)
• Committee member: Jelena Filipović-Grčić (član povjerenstva)
• Committee member: Biserka Cetina-Čižmek (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical technology); Zagreb
• Defense date and country: 2020, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Tehnološko pitanje je bilo mogu li se pripraviti stabilni i kozmetički prihvatljivi temperaturno
osjetjivi hidrogelovi na bazi poloksamera i hijaluronske kiseline s dodatkom ekstrakata biljke
Centella asiatica i/ili ekstrakta crvene alge, Coralline extract. Biološko pitanje je bilo hoće li
hidrogelovi i kako utjecati na hidrataciju kože.
Hidrogelovi su pripravljeni jednostavnom metodom otapanja polimera i dodatka ekstrakata.
Karakterizirani su s obzirom na veličinu čestica i reološka svojstva te je pokazano da se
kombiniranjem 10 o/o poloksamera s 1 o/o hijaluronske kiseline mogu pripraviti stabilni
hidrogelovi čija je kritična temperatura geliranja između 27 i 35 °C te mogu uklapati 5 ili 10 o/o
ekstrakata bez da im se značajno promijeni svojstvo geliranja i viskoznost.
Hidratacijska svojstva hidrogelova su ispitana na koži podlaktice zdravih dobrovoljaca
korištenjem neinvazivne biometrijske metode pomoću MoisturMap MMlOO uređaja. Analizirane
su slike površine kože na koju jesu ili nisu primijenjeni uzorci hidrogelova s obzirom na razinu
sivog (Gray Index).
Rezultati su pokazali da već i sam kombinirani hidrogel poloksamera i hijaluronske kiseline
posjeduje hidratacijska svojstva a najbolja hidratacijska svojstva su pokazali hidrogelovi s
ekstraktom crvene alge i kombinacijom ekstrakta crvene alge i ekstrakta C. asisatice u kojem je
bio prisutan glicerol.
Rezultati predstavljaju doprinos razvoju temperaturno osjetljivih hidrogelova za hidrataciju
kože i normalno funkcioniranje epidermalne barijere.

Abstract (english)

The technological question was whether stable and cosmetically acceptable temperature-sensitive
hydrogels based on poloxamer and hyaluronic acid with the addition of extracts of
Centella asiatica plant and/or red algae extract, Coralline extract could be prepared. The
biological question was whether and how hydrogels affect skin hydration.
Hydrogels were prepared by a simple polymer dissolution method and addition of extracts. They
were characterized by particle size distribution and rheological properties and it has been
demonstrated that by combining 10% of poloxamer and 1 % of hyaluronic acid, stable hydrogels
with a critical gelling temperature between 27 and 35 ° C can be prepared. The addition of S or
10% of the extracts didn't significantly changed their properties of gela ti on and viscosity.
Hidratation hydrogel properties were tested on the forearms of healthy volunteers by noninvasive
biometric methods using MoisturMap devices. Skin images were analyzed with regard
to the level of the gray surface of the skin to which hydrogel samples were or were not applied.
Results have shown that the combined poloxamer and hyaluronic acid hydrogel have hydration
properties and the best hydration properties have been demonstrated by hydrogels with red
algae extracts and a combination of red algae extracts and C. asisatice extract in which glycerol
was present.
The results represent a contribution to the development of temperature-sensitive hydrogels for
skin hydration and norma! functioning of the epiderma! barrier.

• Keywords: hidratacija kože; hidrogelovi; poloksameri; hijaluronska kiselina; ekstrakt crvene alge; ekstrakt Centella asiatice
• Keywords (english): skin hydratation; hydrogels; poloxamers; hyaluronic acid; red algae extract; Centella asiatice
• Language: croatian
• URN:NBN: urn:nbn:hr:163:080847
• Study programme: Title: Dermopharmacy and cosmetology; Study programme type: university; Study level: postgraduate specialist; Academic / professional title: sveučilišni magistar/sveučilišna magistra dermofarmacije i kozmetologije (sveučilišni magistar/sveučilišna magistra dermofarmacije i kozmetologije)
• Type of resource: Text
• File origin: Reformatted digital
• Access conditions: Open access
• Created on: 2021-05-06 13:18:07