Biogena sintena nanočestica selena uz ekstrakt polifenola i pektina iz komine mandarine

master's thesis

• Title (english): Biogenic synthesis of selen nanoparticles using polyphenols and pectin extracted from mandarine peel
• Author: Marta Gosarić
• Mentor: Dubravka Vitali Čepo (mentor)
• Committee member: Dubravka Vitali Čepo (predsjednik povjerenstva)
• Committee member: Ivan Pepić (član povjerenstva)
• Committee member: Petra Turčić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of food chemistry); Zagreb
• Defense date and country: 2020-09-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

U okviru ovog rada istražena je mogućnost korištenja pektina i polifenola ekstrahiranih iz kore
mandarine u biogenoj sintezi nanoselena pri čemu su sirove i pročišćene pektinske frakcije kore
mandarine korištene kao inkapsulacijsko sredstvo dok je polifenolni ekstrakt korišten s namjerom
poboljšanja antioksidativnog potencijala nastalih nanočestica. Nastali nanosustavi karakterizirani su
primjenom difrakcijske laserske spektroskopije te su na taj način određeni promjeri i zeta potencijal
nastalih čestica. Antioksidativna aktivnost čestica određena je Folin-Ciocalteu metodom. Rezultati
provedenih istraživanja pokazuju da se primjenom sirovog pektina izoliranog iz kore mandarine u
koncentracijama od 0.1% i 0.3% mogu sintetizirati stabilne nanosustavi od 232 nm odnosno 292 nm i
zeta potencijalom od -27 .4 odnosno -35. Također, nanočestice selena inkapsulirane sirovim pektinom
izoliranim iz kore mandarine (0.1 i 0.3%) pokazuju značajno veću antioksidativnu aktivnost od kemijski
sintetiziranog nanoselena (2.46 i 1.86 g GAE/mol nSe u odnosu na 0.46 g GAE/mol nSe). Polifenoli iz
ekstrakta kore mandarine adsorbiraju na površinu nastalih nanočestica selena i ostvaruju antioksidativne
učinke, međutim značajno povećavaju promjer nastalih nanočestica i destabiliziraju sustav te se ne mogu
primjenjivati na ovaj način.Buduća istraživanja potrebno je usmjeriti na standardizaciju postupka
ekstrakcije i karakterizaciju pektina iz kore mandarine te optimizaciju izrade polifenolnog ekstrakta,a sa
ciljem dobivanja nešto manjih nanočestica, a većeg antioksidativnog potencijala.

Abstract (english)

The main goal of this thesis was to investigate the possibility of using pectin and polyphenols extracted
from mandarin peel in biogenic synthesis of nanoselen where raw and purified pectin fractions of
mandarin peel were used as an encapsulating agent, while polyphenolic extract was used to improve the
antioxidant potential of nanoparticles. Zeta potential and average diameteres of formed nanoparticles
were determined by laser diffraction spectroscopy. The antioxidant activity of the particles was
determined by the Folin-Ciocalteu method. The results of the conducted research show that by using
crude pectin isolated from mandarin peel in concentrations of 0.1% and 0.3%, stable nanosystems of 232
nm and 292 nm and zeta potential of -27.4mV and -35mV can be synthesized. Also, selenium
nanoparticles encapsulated with crude pectin (0.1 and 0.3%) show significantly higher antioxidant
activity than chemically synthesized nanoselen (2.46 and 1.86 g GAE / mol nSe compared to 0.46 g
GAE / mol nSe). Polyphenols from mandarin peel extract adsorb on the surface of the formed selenium
nanoparticles and contribute to the antioxidant effects. However, they significantly increase the
diameter of the formed nanoparticles and destabilize the system.
Future research needs to focus on standardization of the pectin extraction process and its
characterization as well as optimization of polyphenol extract production, with the aim of obtaining
smaller nanoparticles with higher antioxdiant potential.

• Keywords: kora mandarine; sirovi pektin; polifenolni ekstrakt; antioksidacijsko djelovanje; nanočestice
• Keywords (english): mandarine peel; crude pectin; polyphenol exctract; antioxidative activitiy; nanoparticles
• Language: croatian
• URN:NBN: urn:nbn:hr:163:805146
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-05-13 09:49:17