Fitokemijski sastav i antioksidacijsko djelovanje etanolnih ekstrakata odabranih vrsta roda Artemisia

master's thesis

• Title (english): Phytochemical properties and antioxidant activities of ethanolic extracts of selected Artemisia species
• Author: Marija Canjuga
• Mentor: Maja Bival Štefan (mentor)
• Committee member: Maja Bival Štefan (predsjednik povjerenstva)
• Committee member: Marija Kindl (član povjerenstva)
• Committee member: Jasna Jablan (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy); Zagreb
• Defense date and country: 2019-07-17, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

U okviru ovog diplomskog rada istražen je fitokemijski sastav i antioksidacijski učinak etanolnih ekstrakata listova i grančica odabranih vrsta roda Artemisia. Metodom tankoslojne kromatografije dokazana je prisutnost flavonoida, fenolnih kiselina, seskviterpenskih laktona i diterpena. Ispitane vrste istaknule su se kao bogat izvor fenolnih kiselina. Primjenom spektrofotometrijskih metoda određen je sadržaj flavonoida i fenolnih kiselina u ispitanim etanolnim ekstraktima koji je iznosio je 0,19–0,59 % i 1,74–2,0 %.
Antioksidacijski učinak etanolnih ekstrakata ispitan je različitim spektrofotometrijskim metodama u usporedbi s troloksom i butil-hidroksitoluenom. Istraživanim biljnim ekstraktima ustanovljena je sposobnost hvatanja DPPH radikala (IC50: 13,73–20,25 μg/mL), redukcije iona željeza(III) (IC50: 31,34–47,81 μg/mL) i keliranja iona željeza(II) (IC50: 48,25–152,28 μg/mL). Dobiveni rezultati potvrđuju da su ispitane vrste bogat izvor fenolnih kiselina te da imaju dobru antioksidacijsku aktivnost.

Abstract (english)

The phytochemical composition and the antioxidant activity of ethanolic extracts of leaves and branches of selected Artemisia species (A. alba, A. arborescens, A. absinthium) were studied in this thesis. Flavonoids and phenolic acids were detected with thin-layer chromatography. The content of flavonoids (0,19–0,59 % ) and phenolic acids (1,74–2,0 %.) were determined spectrophotometricaly. The antioxidant activity of the ethanolic extracts was tested using various spectrophotometric methods in comparison with trolox and buthylhydroxytoluene. Tested extracts possessed DPPH free-radical scavenging activity (IC50: 13.73–20.2 μg/mL), ferric reducing ability (IC50: 31.34–47.81 μg/mL) and ferrous chelating activity (IC50: 48.25–152.28 μg/mL). Obtained resuts confirm that Artemisia species are a rich source of phenolic acids and possess antioxidant activity.

• Keywords: Artemisia; fenolne kiseline; antioksidacijski učinak
• Keywords (english): Artemisia; phenolic acid; antioxydant activity
• Language: croatian
• URN:NBN: urn:nbn:hr:163:388791
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-09-07 15:34:17