Određivanje antioksidacijske aktivnosti derivata itakonske kiseline

master's thesis

• Title (english): Determining antioxidant activity of itaconic acid derivatives
• Author: Lucija Škarić
• Mentor: Karmela Barišić (mentor)
• Committee member: Karmela Barišić (predsjednik povjerenstva)
• Committee member: Lidija Bach-Rojecky (član povjerenstva)
• Committee member: Mario Matijašić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of medical biochemistry and haematology); Zagreb
• Defense date and country: 2021-04-09, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Stanice tumora obilježava disbalans osnovnih staničnih regulacijskih mehanizama, a posljedica toga je
njihova nenormalna proliferacija, nekontrolirani rast i dioba. Disbalans oksidacijskih i antioksidacijskih
mehanizama, u korist oksidacije nazivamo oksidacijskim stresom. Tim procesom nastaju slobodni radikali.
Prisutnost slobodnih radikala može uzrokovati lipidnu peroksidaciju, oštećenje DNA i proteina, može
potaknuti citotoksično djelovanje, inducirati mutacije, kromosomske aberacije i naposlijetku dovesti do
razvoja tumora. U ovom je radu ispitivana antioksidacijska aktivnost 10 novosintetiziranih spojeva.
Sintetizirani spojevi derivati su itakonske kiseline i antimalarika primakina, klorokina i meflokina, kao i
konjugati itakonske kiseline i fluoroanilina, piridina i indola. Sintetizirani su kao potencijalni antitumorski
i antivirusni agensi. Odabrana je DPPH metoda kojom se brzo i jednostavno procjenjuje antioksidacijska
aktivnost u složenijim biološkim uzorcima. DPPH metoda uspostavljena je korištenjem dva poznata spoja
s antioksidacijskom aktivnošću, vitaminom C i Troloxom. Potom su standardi i ispitivani spojevi otopljeni
u DMSO otapalu do koncentracije od 50 mM, te su radne otopine pripremljene u etanolu u seriji razrjeđenja
(1 mM - 0,5 nM). Izmjerena im je apsorbancija i izračunata vrijednost antioksidacijske aktivnosti u odnosu
na pozitivnu kontrolu. Ispitivani konjugati itakonske kiseline u DPPH testu nisu pokazali antioksidacijsku
aktivnost. Njihove IC50 vrijednosti bile su veće od 1000 μM.

Abstract (english)

Tumor cells are characterized by the imbalance of basic cellular regulation mechanisms, and the result is
abnormal proliferation, uncontrolled growth, and division. The disbalance between oxidative and
antioxidative mechanisms in favor of oxidation is called oxidation stress. This process creates free radicals.
The presence of free radicals can cause lipid peroxidation, damage to DNA and proteins, stimulation of
cytotoxic actions, induction of mutations, chromosomal aberrations, which will eventually lead to tumor
development. In this paper, the antioxidant activity of 10 newly synthetic compounds were examined.
Synthesized compounds are derivatives of itaconic acid and anti-malarial drugs: primaquine, chloroquine
and mefloquine, as well as conjugates of itaconic acid and fluoroaniline, pyridine and indole. They are
synthesized as potential anticancer and antiviral agents. A DPPH method was chosen to assess antioxidant
activity quickly and easily in more complex biological samples. The DPPH method was established using
two known compounds with antioxidant activity, vitamin C and Trolox. The standards and test compounds
were dissolved in the DMSO solvent to a concentration of 50 mM. The stock solutions were then prepared
in ethanol in a dilute series (1 mM - 0.5 nM). They were measured for absorbance and the value of
antioxidant activity was calculated in relation to positive control. The examined itaconic acid conjugates in
the DPPH test showed no antioxidant activity. Their IC50 values were greater than 1000 μM.

• Keywords: tumori; derivati itakonske kiseline; DPPH metoda; antioksidacijska aktivnost
• Keywords (english): tumours; itaconic acid derivatives; DPPH method; antioxidant activity
• Language: croatian
• URN:NBN: urn:nbn:hr:163:680449
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-11-16 15:59:14