Ispitivanje stabilnosti ekstrakta fekalnog kalprotektina upotrebom komercijalnog sustava za ekstrakciju QUANTA Flash Calprotectin

master's thesis

• Title (english): Stability testing of fecal calprotectin extract using QUANTA Flash Calprotectin commercial extraction system
• Author: Sanja Veltruski
• Mentor: Donatella Verbanac (mentor)
• Committee member: Donatella Verbanac (predsjednik povjerenstva)
• Committee member: Roberta Petlevski (član povjerenstva)
• Committee member: Andrea Tešija Kuna (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of medical biochemistry and haematology); Zagreb
• Defense date and country: 2021-11-03, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Medical Biochemistry

Abstract

Fekalni kalprotektin je važan biomarker za razlikovanje upalnih crijevnih bolesti (IBD, engl. inflamatory bowel disease) od sindroma iritabilnog crijeva (IBS, engl. irritable bowel syndrome), kao i za praćenje tijeka bolesti i recidiva. Metoda je neinvazivna, a pacijent se upućuje na kolonoskopiju u slučaju povišene koncentracije fekalnog kalprotektina odnosno, ukoliko se sumnja na IBD.
U ovom radu ispitana je stabilnost 20 uzoraka tijekom 120 sati. Koncentracija fekalnog kalprotektina se mjerila kemiluminiscentnom imunoanalizom (CLIA) upotrebom komercijalnog sustava za ekstrakciju QUANTA Flash® Calprotectin (INOVA Diagnostics, San Diego, CA, USA) na analizatoru BIO-FLASH (INOVA Diagnostics, San Diego, CA, USA). Zaključno, 85% ekstrakata koji su priređeni i obrađeni po uputama proizvođača zadovoljili su traženim uvjetima stabilnosti.

Abstract (english)

Fecal calprotectin is an essential biomarker for distinguishing inflammatory bowel disease (IBD) from irritable bowel syndrome (IBS) and monitoring the course of disease and relapse. The method is non-invasive, and the patient is referred for colonoscopy in case of elevated fecal calprotectin concentration, particularly if IBD is suspected.
In this experiment, the stability of 20 samples over 120 hours was examined.
Fecal calprotectin concentration was determined by chemiluminescent immunoassay (CLIA) method using a commercial QUANTA Flash® Calprotectin extraction system (INOVA Diagnostics, San Diego, CA, USA) on a BIO-FLASH analyzer (INOVA Diagnostics, San Diego, CA, USA).
Finally, 85% of the extracts prepared and processed according to the manufacturer's instructions meet the required stability requirements.

• Keywords: fekalni kalprotektin; QUANTA Flash Calprotectin; stabilnost; upalne bolesti crijeva; sindrom iritabilnog crijeva
• Keywords (english): fecal calprotectin; QUANTA Flash Calprotectin; stability; inflammatory bowel disease; irritable bowel syndrome
• Language: croatian
• URN:NBN: urn:nbn:hr:163:145645
• Study programme: Title: Medical biochemistry; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra medicinske biokemije i laboratorijske medicine (magistar/magistra medicinske biokemije i laboratorijske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-02-09 10:39:01