Određivanje flavonoida u komercijalnim uzorcima kamiličinog cvijeta

master's thesis

• Title (english): Determination of flavonoids in commercial samples of chamomile flower
• Author: Lucia Mahovlić
• Mentor: Biljana Blažeković (mentor)
• Committee member: Biljana Blažeković (predsjednik povjerenstva)
• Committee member: Maja Bival Štefan (član povjerenstva)
• Committee member: Miranda Sertić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy); Zagreb
• Defense date and country: 2021-09-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Ljekovita biljna droga Matricariae flos ima duge tradiciju primjene u europskoj fitoterapiji, i jedan je od najčešće korištenih čajeva na hrvatskom tržištu. Među širokim spektrom farmakološki i klinički dokazanih učinaka posebno je cijenjena zbog protuupalnih i spazmolitičkih svojstava. Terapijski učinak droge uglavnom se pripisuje sastavnicama eteričnog ulja, poput kamazulena i α-bisabolola, te flavonoidima među kojima je najznačajniji apigenin. U okviru ovog diplomskog rada analizirana su 22 uzorka kamiličinog cvijeta sakupljena na hrvatskom tržištu tijekom 2019. godine koji su obuhvaćali biljnu drogu Matricariae flos dostupnu u in toto obliku i usitnjenu drogu u obliku filter vrećica. Provedena je kvantitativna analiza flavonoida spektrofotometrijskom metodom i kvalitativna analiza flavonoida metodom tankoslojne kromatografije. Uzorci in toto kamiličinog cvijeta sadržavali su između 0,40 % i 0,51 % flavonoida, dok je njihov udio u uzorcima droge dostupne u filter vrećicama bio bitno neujednačeniji i kretao se u širokom rasponu od 0,16 % do 0,43 %. Općenito je utvrđeno da su cjelovite cvjetne glavice kamilice znatno bogatiji izvor flavonoida u odnosu na usitnjenu drogu prisutnu u obliku filter vrećica. Dobiveni rezultati ukazuju na značajne razlike u sadržaju ljekovitih tvari među različitim oblicima kamiličinog cvijeta i ukazuju na važnost kontrole kakvoće uzoraka sa tržišta kao i jačanje postojeće zakonske regulative u području hrane i dodataka prehrani vezano za ljekovite biljne droge.

Abstract (english)

The herbal drug Matricariae flos has a long tradition of use in European phytotherapy and is one of the mostly used teas on the Croatian market. Among the wide range of pharmacologically and clinically proven effects, it is especially valued for its anti-inflammatory and antispasmodic properties. The therapeutic effect of the drug is mainly attributed to the components of the essential oil, such as kamazulene and α-bisabolol, and flavonoids, among which the most important is apigenin. Within this diploma thesis, 22 samples of chamomile flower collected on the Croatian market during 2019 were analyzed, which included the herbal drug Matricariae flos available in in toto form and crushed drug in the form of filter bags. Quantitative analysis of flavonoids by spectrophotometric method and qualitative analysis of flavonoids by thin layer chromatography were performed. Samples of in toto chamomile flower contained between 0.40% and 0.51% of flavonoids, while their share in drug samples available in filter bags was significantly more uneven and ranged widely from 0.16% to 0.43%. It has generally been found that whole chamomile flower heads are a much richer source of flavonoids than the crushed drug present in the form of filter bags. The obtained results indicate significant differences in the content of medicinal substances among different forms of chamomile flower and indicate the importance of quality control of samples from the market as well as strengthening the existing legislation in the field of food and food supplements related to medicinal herbal drugs.

• Keywords: flavonoidi; Matricariae flos; kamiličin cvijet; TLC; spektrofotometrija
• Keywords (english): flavonoids; Matricariae flos; chamomile flower; TLC; spectrophotometry
• Language: croatian
• URN:NBN: urn:nbn:hr:163:287989
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-02-09 13:35:12