Biološka aktivnost polipropilenglikolnih ekstrakata zeleni hmeljaste vije (Medicago lupulina L.)

master's thesis

• Title (english): Biological activities of black medic (Medicago lupulina L.) aerial parts extracts in polypropylene glycol
• Author: Anamaria Blažević
• Mentor: Marijana Zovko Končić (mentor)
• Committee member: Marijana Zovko Končić (predsjednik povjerenstva)
• Committee member: Ivan Pepić (član povjerenstva)
• Committee member: Marijan Marijan (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy); Zagreb
• Defense date and country: 2021-06-15, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Starenje kože složen je biološki proces koji se ubrzava pod utjecajem oksidativnog stresa uzrokovanog različitim nepovoljnim okolišnim čimbenicima. Kako bi se umanjio utjecaj oksidativnog stresa na starenje kože, savjetuje se primjena kozmetičkih proizvoda bogatih antioksidansima. Obzirom da sintetski antioksidansi često uzrokuju neželjene reakcije, poput iritacijskog kontaktnog dermatitisa, sve se više istražuju antioksidativni učinci raznih biljnih ekstrakata. Cilj ovog istraživanja bio je spektrofotometrijski odrediti sadržaj ukupnih polifenola, flavonoida i fenolnih kiselina u polipropilenglikolnim ekstraktima zeleni vrste M. lupulina. U svrhu potencijalne primjene navedenih ekstrakata u kozmetičkim proizvodima, ispitana je njihova antiradikalna aktivnost korištenjem DPPH metode, sposobnost keliranja Fe2+ iona te inhibitorni učinak na tirozinazu. Istraživanje je pokazalo da je ekstrakt dobiven korištenjem 10 %-tnog polipropilenglikola kao ekstrakcijskog otapala bogatiji ispitivanim spojevima te pokazuje bolju kelirajuću aktivnost od ekstrakta dobivenog korištenjem 50 %-tnog polipropilenglikola. Ekstrakti su pokazali relativno slab antiradikalni i kelirajući učinak. Ekstrakt bogatiji fenolnim sastavnicama bio je slabiji hvatač slobodnih radikala, ali je pokazao bolju kelirajuću aktivnost. Ekstrakti su pokazali slabo inhibitorno djelovanje na tirozinazu. Provedena istraživanja pokazala su stanoviti potencijal ove biljne vrste za primjenu u kozmetici, ali za donošenje konačnih zaključaka potrebno je provesti detaljnije studije.

Abstract (english)

Skin aging is a complex biological process that is accelerated under the influence of oxidative stress caused by different unfavourable environmental factors. To reduce the influence of oxidative stress on skin aging, cosmetic products rich in antioxidants are recommended to be used. Bearing in mind that synthetic antioxidants often cause adverse reactions such as irritable contact dermatitis, newer research is based on exploring antioxidative effects of different plant extracts. The aim of this study was to spectrophotometrically determine the total phenolic, flavonoid and phenolic acids content in polypropylene glycol extracts of M. lupulina aerial parts. Furthermore, to determine the potential use of these extracts in cosmetic products, their antiradical activity was investigated using DPPH method, as well as Fe2+-chelating activity and tyrosinase inhibitory activity. The tested extracts showed a rather low antiradical and chelating activity. The extract richer in phenolic components was a weaker scavenger of free radicals but showed better chelating activity. Extracts have shown poor inhibitory effects on tyrosinase. Research has shown a certain potential of this plant species for use in cosmetics, but more detailed studies need to be carried out to draw definitive conclusions.

• Keywords: hmeljasta vija; polipropilenglikol; ukupni polifenoli; flavonoidi; fenolne kiseline; antioksidativna aktivnost; tirozinaza
• Keywords (english): black medic; polypropylene glycol; total phenols; flavonoids; phenolic acids; antioxidative activity; tyrosinase
• Language: croatian
• URN:NBN: urn:nbn:hr:163:130121
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2023-06-15
• Created on: 2022-03-01 10:56:43