Otpornost mikobiote lanenog platna na gama zračenje

master's thesis

• Title (english): Resistance of linen canvas mycobiota to gamma irradiation
• Author: Silvija Marjanović
• Mentor: Maja Šegvić Klarić (mentor)
• Committee member: Maja Šegvić Klarić (predsjednik povjerenstva)
• Committee member: Daniela Jakšić (član povjerenstva)
• Committee member: Ana-Marija Domijan (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of microbiology); Zagreb
• Defense date and country: 2021-04-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Veliki dio kulturne baštine čine predmeti od organskog, biljnog materijala, platna. Kao takvi izrazito su podložni kontaminaciji gljivicama koje svojim djelovanjem ubrzavaju njegovo propadanje. Jedna od najpogodnijih metoda za zaustavljanje destruktivnog djelovanja gljivica je gama zračenje. U ovom radu ispitivana je otpornost mikobiote lanenog platna na gama zračenje, te otpornost inokuliranog sekundarnog kolonizatora, vrste Cladosporium sphaerospermum. Uzorci su tretirani pomoću izvora u Laboratoriju za radijacijsku kemiju i dozimetriju (LRKD) Instituta Ruđer Bošković (IRB). Primijenjene su apsorpcijske doze od 2, 7, 20 i 50 kGy u dvije brzine zračenja 0,1 Gy/s i 8,6 Gy/s. Mikrobiološka analiza ozračenih uzoraka provedena je neposredno nakon zračenja te 14., 28., i 56. dan nakon zračenja.
Prirodnu mikobiotu platna čine vrste iz rodova Penicillium, Cladosporium, Fusarium i kvasci. Antifungalni učinak gama zračenja ovisan je o dozi i brzini doze. Vrste Fusarium spp., narančasti kvasci i Cladosporium spp. pokazali su se najrezistentnijim gljivicama na gama zračenje. Iz rezultata pokusa određena je i D10 vrijednost od 13,72 kGy za višu brzinu zračenja (8,6 Gy/s). Potrebno je provesti kemijsku analizu platna kako bi se dobio bolji uvid u utjecaj određenih doza i brzina zračenja na sastav platna. Za učinkovitu primjenu gama zračenja u konzervaciji platnenih predmeta kulturne baštine potreban je interdisciplinarni pristup.

Abstract (english)

Major part of the cultural heritage consists of objects of organic, plant material, canvas. As such, they are highly susceptible to fungal contamination, which by its action accelerate the deterioration of the canvas. One of the most suitable methods to stop the destructive action of fungi is gamma radiation. This paper investigates the resistance of linen mycobiota to gamma radiation as well as the resistance of the inoculated secondary colonizer type Cladosporium sphaerospermum. The samples were treated with a source at the Laboratory for radiation chemistry and dosimetry of Ruđer Bošković Institute. Absorption doses of 2, 7, 20 and 50 kGy were applied at two radiation rates of 0,1 and 8,6 Gy/s. Microbiological analysis of irradiated samples was carried out immediately after radiation and on the 14th, 28th and 56th day after radiation.
Natural mycobiota of linen material includes species from the genera Penicillium, Cladosporium, Fusarium and yeast. The antifungal effect of gamma radiation is dependent on dose and dose rate. Fusarium spp., orange yeasts and Cladosporium spp. proved to be the most resistant to gamma radiation. The test results also set D10 value of 13,72 kGy for higher radiation rate (8,6 Gy/s). A chemical analysis of the linen material should be carried out in order to obtain better insight into the impact of certain doses and the radiation rates on the composition of the canvas. An interdisciplinary approach is required for the effective application of gamma radiation in the conservation of canvas objects of cultural heritage.

• Keywords: kulturna baština; gama zračenje; plijesni; Cladosporium
• Keywords (english): cultural heritage; gamma irradiation; moulds; Cladosporium
• Language: croatian
• URN:NBN: urn:nbn:hr:163:708396
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2022-04-29
• Created on: 2022-03-04 13:48:50