Analiza abemacikliba, ribocikliba i palbocikliba zonskom kapilarnom elektroforezom

master's thesis

• Title (english): Analysis of abemaciclib, ribociclib and palbociclib using capillary zone electrophoresis
• Author: Iva Begović
• Mentor: Miranda Sertić (mentor)
• Committee member: Miranda Sertić (predsjednik povjerenstva)
• Committee member: Biljana Nigović (član povjerenstva)
• Committee member: Maja Ortner Hadžiabdić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical analysis); Zagreb
• Defense date and country: 2021-07-01, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Zbog sve veće pojavnosti raka dojke u populaciji, postoji potreba za razvijanjem analitičkih metoda koje će moći koristiti u terapijskom praćenju lijekova kako bi se pospješio uspjeh terapije.
Cilj ovog istraživanja bio je razvoj nove kapilarnoelektroforetske metode kako bi se na jednostavan, brz i točan način mogao detektirati ili kvantificirati lijekove iz skupine cikliba – abemaciklib, ribociklib ili palbociklib. Korištena tehnika je zonska kapilarna elektroforeza.
Korištena je kapilara od izvučenog kvarca duljine 32,5 cm unutrašnjeg promjera 50 μm. Uzorci su u kapilaru injektirani pod tlakom od 50 mbar, pri temperaturi 25 °C tijekom 6 s. Valna duljina na kojoj su provođene analize iznosi 320 nm.
Optimizirani su sljedeći parametri - vrsta i pH pufera, koncentracija pufera, napon, temperatura i uvjeti ispiranja. Pratio se njihov utjecaj na vrijeme analize, površinu pika, broj teorijskih tavana i simetriju pika. Kao optimalne vrijednosti uzete su 50 mM fosfatni pufer pH 2,5, napon 27,5 kV i temperatura 30 °C. Za optimalne uvjete ispiranja odabrano je da se na početku dana ispire 10 min s 10% fosfornom kiselinom, 10 min s ultračistom vodom i 20 min s radnim puferom, a na kraju dana 20 min s ultračistom vodom te da se između mjerenja radi prekondicioniranje 5 min s radnim puferom. Pokazalo se da su za uspješnu i reproducibilnu analizu dostatni i uvjeti rada s citratnim puferom pri pH 3,17, koncentracije pufera 113 mM, temperature 25 °C, napona 20 kV i pH 4,11, koncentracije pufera 35 mM, temperature 25 °C i napona 25 kV.

Abstract (english)

Considering the growing incidence of breast cancer in our population, there is a need to develop an analytical method that can be used in therapeutic drug monitoring to enhance the success of therapy.
The goal of this research was to develop a new capillary electrophoresis method so that any of the observed drugs from the ciclib group – abemaciclib, ribociclib or palbociclib could be detected or quantified in a simple, fast and accurate way. The technique used is zone capillary electrophoresis.
This analysis was performed on a 32,5 cm long capillary of extracted quartz with an inner diameter of 50 μm. The samples were injected into the capillary under a pressure of 50 mbar, at a temperature of 25 °C for 6 seconds. A wavelength of 320 nm was used.
The following parameters were optimized through the study - type and pH of the buffer, buffer concentration, voltage, temperature and flushing conditions. Their influence on the analysis time, peak area, number of theoretical plates and peak symmetry was monitored. The optimal values found included 50 mM phosphate buffer, voltage 27.5 kV and temperature 30 °C. For optimal flushing conditions, it was chosen to flush at the beginning of the day for 10 minutes with 10% phosphoric acid, 10 minutes with ultrapure water and 20 minutes with the working buffer, and at the end of the day 20 minutes with ultrapure water and to precondition for 5 minutes between measurements with the working buffer. It turned out that for the successful and reproducible analysis, the working conditions with the citat buffer at pH 3.17, buffer concentration 113 mM, temperature 25 °C, voltage 20 kV and pH 4.11, buffer concentration 35 mM, temperature 25 °C and voltage 25 kV were also sufficient.

• Keywords: abemaciklib; ribociklib; palbociklib; kapilarna elektroforeza; rak dojke
• Keywords (english): abemaciclib; palbociclib; ribociclib; capillary electrophoresis; breast cancer
• Language: croatian
• URN:NBN: urn:nbn:hr:163:797679
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2023-07-01
• Created on: 2022-03-29 10:21:13