Biološka aktivnost glicerolnih ekstrakata zeleni hmeljaste vije (Medicago lupulina L.)

master's thesis

• Title (english): Biological activity of black medick (Medicago lupulina L.) glycerol extracts
• Author: Mateja Horvat
• Mentor: Marijana Zovko Končić (mentor)
• Committee member: Marijana Zovko Končić (predsjednik povjerenstva)
• Committee member: Maja Bival Štefan (član povjerenstva)
• Committee member: Gordana Maravić Vlahoviček (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy); Zagreb
• Defense date and country: 2022-04-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Oksidativni stres ima važnu ulogu u procesu starenja kože i razvoju kroničnih bolesti povezanih sa
starenjem. S obzirom da polifenoli imaju snažno antioksidativno djelovanje te smanjuju učinak
oksidativnog stresa na starenje kože, česti su sastojci dermatoloških i kozmetičkih proizvoda. Cilj
istraživanja bio je spektrofotometrijski odrediti sadržaj ukupnih polifenola, flavonoida te fenolnih
kiselina u glicerolnim ekstraktima zeleni hmeljaste vije. Kako bi se utvrdilo potencijalno korištenje
ekstrakata u topičkim pripravcima, korištene su metode za određivanje antioksidativne aktivnosti.
Ispitana je antiradikalna aktivnost korištenjem DPPH reagensa te kelirajuća aktivnost prema Fe2+
ionima. Biološka aktivnost inhibicije tirozinaze u svrhu potencijalnog korištenja biljke protiv
poremećaja pigmentacije na koži ispitana je enzimskom metodom. Istraživanje je pokazalo da
ekstrakt pripremljen u 60 %-tnom glicerolu kao ekstrakcijskom otapalu ima bolju kelirajuću
aktivnost te je bogatiji fenolnim kiselinama u odnosu na ekstrakt pripremljen u 90 %-tnom glicerolu.
Ekstrakt pripremljen u 90 %-tnom glicerolu nešto je bogatiji flavonoidima te ima bolje antiradikalno
djelovanje. Ekstrakti su pokazali zanemarivo inhibirajuće djelovanje na tirozinazu. S obzirom na
relativno dobru antioksidativnu aktivnost, M. lupulina ima potencijal za korištenje u dermatološke i
kozmetičke svrhe, no potrebna su daljnja istraživanja kako bi se utvrdile njezine značajke u odnosu
na druge biljne vrste koje se koriste u kozmetičke svrhe.

Abstract (english)

Oxidative stress plays an important role in the aging process of the skin and the development of
chronic diseases associated with aging. Since polyphenols have a strong antioxidant effect and
reduce the effect of oxidative stress on skin aging, they are common ingredients in dermatological
and cosmetic products. The aim of the study was to spectrophotometrically determine the content of
total polyphenols, flavonoids, and phenolic acids in glycerol extracts of black medick aerial parts.
Methods for determining antioxidant activity were used to determine the potential use of extracts in
skin products. Antiradical activity using DPPH reagent and chelating activity against Fe2+ ions were
investigated. The biological activity of tyrosinase inhibition for the potential use of plant against
skin pigmentation disorders was investigated by an enzymatic method. The study showed that the
extract prepared in 60 % glycerol as an extraction solvent has better chelating activity and is richer
in phenolic acids compared to the extract prepared in 90 % glycerol. The extract prepared in 90 %
glycerol is slightly richer in flavonoids and has better antiradical effect. The extracts showed
negligible inhibitory activity on tyrosinase. Due to its relatively good antioxidant activity, M.
lupulina has the potential to be used for dermatological and cosmetic purposes, but further research
is needed to assess its activity in comparison with other species that are used for cosmetic purposes.

• Keywords: hmeljasta vija; glicerol; polifenoli; flavonoidi; fenolne kiseline; antioksidativna aktivnost; tirozinaza
• Keywords (english): black medick; glycerol; polyphenols; flavonoids; phenolic acids; antioxidant activity; tyrosinase
• Language: croatian
• URN:NBN: urn:nbn:hr:163:827309
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-05-26 12:54:29