Antikancerogena aktivnost nanočestica selena stabiliziranih pektinima rajčice i mandarine

master's thesis

• Title (english): Anticancer activity of selenium nanoparticles synthesized with tomato and tangerine pectins as a coating agent
• Author: Lucija Tesla
• Mentor: Dubravka Vitali Čepo (mentor)
• Committee member: Dubravka Vitali Čepo (predsjednik povjerenstva)
• Committee member: Petra Turčić (član povjerenstva)
• Committee member: Kristina Radić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of food chemistry); Zagreb
• Defense date and country: 2022-07-04, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

U ovom istraživanju razvijeni su postupci biosinteze nanoselena uz stabilizaciju pektinima rajčice i
mandarine kao sredstava za oblaganje te uz dodatnu funkcionalizaciju ekstraktom komine masline
(EKM) bogatim polifenolima. Nakon provedene sinteze, provedena je fizikalno-kemijska
karakterizacija dobivenih nanosustava te je ispitan njihov učinak na stanične linije HepG2 i Caco-2.
Dodatkom EKM povećan je prinos sinteze, a nanočestice su pokazale unimodalni tip raspodjele s
rasponom veličine srednjeg promjera 110,4 do 235,4 nm. Zeta potencijal novosintetiziranih sustava
kretao se u rasponu od -25, 9 mV do -21,1 mV, gdje su negativnije vrijednosti dobivene u
funkcionaliziranim nanočesticama. IC50 vrijednosti sintetiziranih nanosustava kretale su se 56.4-
245.2 µg/mL. Selen u nanoobliku pokazao je manju toksičnost od selenita, a uzorci funkcionalizirani
EKM uglavnom su pokazali manju toksičnost u odnosu na nefunkcionalizirane uzorke. Primjenom
pektina komine mandarine i rajčice moguće je dobiti nanočestice selena, smanjene toksičnosti (u
odnosu na selenit) i odgovarajućih fizikalno-kemijskih i funkcionalnih svojstava, uključujući i
antikancerogeno djelovnje, koja se dodatno poboljšavaju funkcionalizacijom ekstrakta komine
masline.

Abstract (english)

In this paper, we investigated the possibility of nanoselenium biosynthesis using tomato- and
mandarin pomace derived pectins as coating agents and olive pomace extract (OPE) as the
functionalization agent. The relative yields of the newly propesed synthesis processes were
examined by UV / VIS spectroscopy; the size and stability of the nanoparticles were determined by
using the dynamic light scattering technique and by measuring zeta potential. The anticancer efects
of obtained nanoparticles were investigated in HepG2 and Caco-2 cell lines. using the colorimetric
MTT method, and the IC50 value was calculated for each sample. Using these methods, it was
determined that unimodal particle size distribution was obtained by proposed biosynthesis processes
with average diameters rangin from 110.4 to 235.4 nm. Zeta potential of investigated nanosystems
ranged from -25.9 mV to -21.1 mV and therefore all obtained samples can be considered stable.
Samples fuctionalized with OPE showed lower negative values of zeta potential indicating improved
stability. IC50 values for the HepG2 cell line showed that all nanoselenium samples had significantly
lower toxicity in comparison to the inorganic form of selenium, selenite. Similar was shown on
Caco-2 cell culture with the exception of a sample synthesized with purified tomato pectin and with
the addition of OPE that showed higher toxicity than selenite. In conclusion, with this type of
biosynthesis, it is possible to synthesize stable selenium nanoparticles that show less toxicity in
comparison to selenite.and posses anticancer effects that are aditionally improved by
functionalization by OPE.

• Keywords: nanoselen; pektin; komina mandarine; komina rajčice; antikancerogeno djelovanje; stanična linija HepG2; stanična linija Caco-2
• Keywords (english): nanoselenium; tangerine pomace; tomato pomace; anticancer properties; HepG2 cell line; Caco-2 cell line
• Language: croatian
• URN:NBN: urn:nbn:hr:163:320161
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-07-12 12:47:54