Fitokemijski sastav i antioksidativno djelovanje ploda drijena (Cornus mas L.)

master's thesis

• Title (english): Phytochemical composition and antioxidant activity of cornelian cherry fruit (Cornus mas L.)
• Author: Ivana Miholić
• Mentor: Biljana Blažeković (mentor)
• Committee member: Biljana Blažeković (predsjednik povjerenstva)
• Committee member: Hrvoje Rimac (član povjerenstva)
• Committee member: Maja Bival Štefan (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy); Zagreb
• Defense date and country: 2022-07-21, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

U okviru ovog diplomskog rada istraženi su fitokemijski sastav i antioksidacijska svojstva
ploda vrste Cornus mas. Metodom tankoslojne kromatografije u drogi je dokazana prisutnost
fenolnih kiselina i antocijana. Spektrofotometrijskim metodama određeno je da ekstrakt
drijenovog ploda sadrži 0,32 % fenolnih kiselina, 0,06 % antocijana, 0,21 % trjeslovina te
0,03% flavonoida. Antioksidacijski potencijal ekstrakta drijenovog ploda vrednovan je na
temelju sposobnosti hvatanja slobodnih radikala i uspoređen s aktivnošću referentnih
antioksidansa (askorbinska kiselina i kvercetin). Utvrđeno je da etanolno-vodeni ekstrakt
droge posjeduje sposobnost hvatanja slobodnih DPPH radikala te ostvaruje 50%-tnu
učinkovitost pri koncentraciji 76,14 μg/mL. Za razliku od čistih antioksidansa, ispitivani
ekstrakt drijenovog ploda nije pokazao značajnu sposobnost hvatanja NO radikala. Dobiveni
rezultati doprinose znanstvenim spoznajama o bioaktivnim sastavnicama i antioksidacijskom
potencijalu drijena koji raste u Hrvatskoj.

Abstract (english)

In this work, phytochemical composition and antioxidant properties of Cornus mas fruit were
studied. Thin-layer chromatography revealed the presence of phenolic acids and anthocyanins
in the studied fruit. It was determined by spectrophotometric methods that the cornelian
cherry fruit extract contains 0.32% phenolic acids, 0.06% anthocyanins, 0.21 % tannins and
0.03% flavonoids. The antioxidant potential of the extract was evaluated based on the ability
to scavenge free radicals and was compared with the activity of reference antioxidants
(ascorbic acid and quercetin). It was established that the cornelian cherry fruit extract posses
the ability to scavenge DPPH free radicals and achieves 50% efficiency at concentration
7 6.14 μg/mL. In contrast to reference antioxidants, the investigated plant extract did not show
significant ability to scavenge NO radicals. The obtained results contribute to scientific
knowledge on the bioactive compounds and antioxidant potential of cornelian cherry fruit
originating from Croatia.

• Keywords: Cornus mas L.; drijenov plod; Corni mas fructus; antioksidativna aktivnost; fenolne kiseline; polifenoli
• Keywords (english): Cornus mas L.; cornelian cherry fruit; ethanol extract of cornelian cherry fruit; antioxidant activity; phytochemical compounds
• Language: croatian
• URN:NBN: urn:nbn:hr:163:780086
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2024-07-21
• Created on: 2022-08-26 11:12:02