Optimizacija kapilarnoelektroforetske metode za analizu letrozola

master's thesis

• Title (english): Optimisation of a capillary electrophoretic method for letrozole analysis
• Author: Kristina Matijašić
• Mentor: Miranda Sertić (mentor)
• Committee member: Miranda Sertić (predsjednik povjerenstva)
• Committee member: Biljana Nigović (član povjerenstva)
• Committee member: Tajana Silovski (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical analysis); Zagreb
• Defense date and country: 2022-07-22, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Zbog velike učestalosti raka dojke i razvoja različitih terapijskih mogućnosti liječenja ove bolesti, od iznimne je važnosti razvoj novih analitičkih metoda za terapijsko praćenje liječenja odabranim lijekovima.
Cilj je ovog istraživanja bio razviti brzu i jednostavnu kapilarnoelektroforetsku metodu za analizu letrozola koja je ekološki prihvatljivija i jeftinija od postojeće HPLC metode. Kako je letrozol u radnim uvjetima analize nenabijen, kao prikladna tehnika odabrana je micelarna elektrokinetička kromatografija.
Analiza je provedena na kapilari od izvučenog kvarca duljine 35 cm, duljine puta do detektora od 27 cm i unutrašnjeg promjera 50 μm. Uzorci su hidrodinamički injektirani u kapilaru pod tlakom od 50 mbar, u vremenu od 4 sekunde. Za detekciju letrozola korištena je valna duljina od 200 i 219 nm.
Tijekom optimizacije metode ispitan je utjecaj koncentracije boratnog pufera (15 – 25 mM) i SDS-a (20 – 65 mM) te temperature (20 – 30 °C) na vrijeme analize i površinu pika. Kao optimalne vrijednosti pokazale su se 15 mM boratni pufer pH 9,3 uz dodatak 35 mM SDS-a.
U daljnjim istraživanjima bit će dodan unutarnji standard kako bi se izbjegli vanjski utjecaji na rezultat analize.

Abstract (english)

Due to high incidence of breast cancer in our population and the developement of new treatment options for this desease it is extremly important to work on deveoping new analytical methods for therapeutic drug monitoring .
The aim of this study was to develop a fast and simple capillary electrophoretic method for the analysis of letrozole, which will also be more environmentally friendly and cheaper than the already known HPLC method. In working environment letrozole is neutral so the best option for his analysis was micellar electrocinetic cromathography.
The analysis was performed on a 35 cm long capillary of fused silica with effective lenght of 27 cm and an innner diameter of 50μm. For the detection of letrozole we used a wavelenghts of 200 and 219 nm.
During the optimisation of method we observed the effect of the concentration of borate buffer (15-25 mM) and SDS (20-65 mM), as well as temperature (20-30 °C) on the migration time and peak area. Considering the experimental results, 15 mM borate buffer, 35 mM SDS and a temperature of 20 °C were taken as optimal values. In further developement of method, it is required to use internal standard in order to eliminate any external effect on the analysis.

• Keywords: letrozol; kapilarna elektorforeza; rak dojke
• Keywords (english): letrozole; capillary electrophoresis; breast cancer
• Language: croatian
• URN:NBN: urn:nbn:hr:163:649956
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2024-07-22
• Created on: 2022-08-30 07:45:43