Interakcije prehrambenih vlakana s karotenoidima i kurkuminom iz biljnih ekstrakata: utjecaj na biodostupnost

master's thesis

• Title (english): Interaction of dietary fibers with carotenoids and curcumin from plant extracts: effect on bioavailability
• Author: Zrinka Filić
• Mentor: Dubravka Vitali Čepo (mentor)
• Committee member: Dubravka Vitali Čepo (predsjednik povjerenstva)
• Committee member: Kristina Radić (član povjerenstva)
• Committee member: Petra Turčić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of food chemistry); Zagreb
• Defense date and country: 2022-09-12, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Beta karoten, likopen i kurkumin su biljni pigmenti koji pokazuju veliki antioksidacijski potencijal. Bitni su i često korišteni u održavanju ljudskog zdravlja i sprječavanju nastanka bolesti. Zbog svog antioksidacijskog djelovanja smatraju se nutraceuticima – vrijednim sastavnicama novih oblika enteralne prehrane.
Enteralna prehrana, zbog svoje uloge dopunjavanja ljudske prehrane u stanjima i bolestima kada je to kompromitirano kroz zdravu prehranu, sadržajno je bogata makronutrijentima i mikronutrijentima. Prehrambena vlakna su bitna sastavnica koja osigurava zdravu probavu i može djelovati probiotički, no zbog svojih fizikalno-kemijskih svojstava stupaju u interakcije s karotenoidima i kurkuminom te mogu smanjiti njihovu biodostupnost.
U ovom diplomskom radu ispitana je stabilnost beta karotena, likopena i kurkumina u simuliranim uvjetima probave te biodostupnost s 10 mješavinama prehrambenih vlakana.
Beta karoten i likopen pokazali su stabilnost pri simulaciji uvjeta probave, dok se koncentracija kurkumina značajno smanjila (na oko 70%).
Likopenu je svih 10 ispitivanih mješavina prehrambenih vlakana smanjilo biodostupnost, dok je biodostupnost beta karotena i kurkumina s nekim mješavinama ostala nepromijenjena. Dvije standardne mješavine STAND-2 i STAND-3 imaju značajan utjecaj na smanjenje biodostupnosti sva tri ispitivana spoja. Inovativne mješavine imaju različiti utjecaj na spojeve, ovisno o njihovom sastavu. Sve inovativne mješavine imaju mali utjecaj na biodostupnost beta karotena. Najveća biodostupnost likopena je s mješavinom INOV-1B (98,3%). Najveća biodostupnost kurkumina je uz mješavinu STAND-1 (113,7%). Dobiveni rezultati upućuju na sličnost dviju standardnih mješavina (STAND-2 i STAND-3), a različite sastave ostalih 8 mješavina te različite afinitet prema vezanju ispitivanih spojeva ovisno o sastavu mješavina.

Abstract (english)

Beta carotene, lycopene and curcumin are plant pigments that show great antioxidant potential. They are important in maintaining human health and preventing diseases. Due to their antioxidant effect, they are considered nutraceuticals - valuable components of new formulas of enteral nutrition.
Enteral nutrition, due to its role of supplementing human nutrition in conditions and diseases that compromise ingestion through a healthy diet, is rich in macronutrients and micronutrients. Dietary fibers are an essential component that ensures healthy digestion and can have a probiotic effect, but due to its physical and chemical properties, its interaction with carotenoids and curcumin can reduce their bioavailability.
The stability of beta carotene, lycopene, and curcumin in simulated digestive conditions and their bioavailability as affected by different dietary fibers mixtures were examined in this master thesis.
Beta carotene and lycopene showed stability during the simulation of digestion conditions, while the concentration of curcumin decreased significantly (to about 70%).
The bioavailability of lycopene was reduced by all 10 dietary fiber mixtures that were analyzed, while the bioavailability of beta-carotene and curcumin remained unchanged when analyzed with some of 10 mixtures. Two standard mixtures STAND-2 and STAND-3 have a significant effect on reducing the bioavailability of all three analyzed compounds. Innovative mixtures have different effects on compounds, depending on their composition. All innovative mixtures have little effect on the bioavailability of beta-carotene. The highest bioavailability of lycopene was shown with the mixture INOV-1B (98.3%). The highest bioavailability of curcumin was shown with the STAND-3 mixture (113.7%). With obtained results it can be concluded there is similarity between two standard mixtures (STAND-2 and STAND-3), and there is difference between composition of the other 8 mixtures with different affinity towards the binding of analyzed compounds.

• Keywords: karotenoidi; beta karoten; likopen; kurkumin; stabilnost; biodostupnost; prehrambena vlakna
• Keywords (english): carotenoids; beta carotene; lycopene; curcumin; stability; bioavailability; dietary fibers
• Language: croatian
• URN:NBN: urn:nbn:hr:163:434462
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-09-12 13:42:58