Polifenolne sastavnice i antioksidacijsko djelovanje droge Myrtilli fructus siccus

master's thesis

• Title (english): Polyphenolic compounds and antioxidant activity of Myrtilli fructus siccus
• Author: Barbara Martinko
• Mentor: Biljana Blažeković (mentor)
• Committee member: Biljana Blažeković (predsjednik povjerenstva)
• Committee member: Maja Bival Štefan (član povjerenstva)
• Committee member: Ana-Marija Domijan (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy); Zagreb
• Defense date and country: 2022-09-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

U okviru ovog diplomskog rada istražen je fitokemijski sastav i antioksidacijska svojstva suhog ploda samonikle borovnice (Vaccinium myrtillus L., Ericaceae) koja raste u Hrvatskoj, te njenog vodenog-etanolnog ekstrakta. Metodom tankoslojne kromatografije u borovničinom plodu je dokazana prisutnost polifenolnih sastavnica. Spektrofotometrijskim metodama određen je sadržaj trjeslovina, fenolnih kiselina, antocijana i flavonoida u plodu i vodeno-etanolnom ekstraktu. Antioksidacijska aktivnost vodeno-etanolnog ekstrakta suhog borovničinog ploda procjenjena je na temelju sposobnost hvatanja NO i DPPH radikala i uspoređena sa učinkom standardnih antioksidansa. Utvrđeno je da je plod borovnice bogat izvor polifenolnih spojeva, posebice antocijana, i posjeduje antioksidativna svojstva. Dobiveni rezultati doprinose znanstvenim spoznajama o fitokemijskom sastavu i biološkoj aktivnosti droge Myrtilli fructus siccus hrvatskog porijekla.

Abstract (english)

In this thesis, the phytochemical composition and antioxidant properties of the dry fruit of wild bilberry (Vaccinium myrtillus L., Ericaceae) growing in Croatia, and its water-ethanol extract, were investigated. The presence of polyphenolic components in the bilberry fruit was proven by the method of thin-layer chromatography. The contents of tannins, phenolic acids, anthocyanins and flavonoids in the fruit and water-ethanol extract were determined by appropriate spectrophotometric methods. The antioxidant activity of the water-ethanol extract of dry bilberry fruit was evaluated based on the ability to scavenge NO and DPPH radicals and compared with the effect of standard antioxidants. It has been established that the bilberry fruit is a rich source of polyphenolic compounds, especially anthocyanins, and has antioxidant properties. The obtained results contribute to scientific knowledge on the phytochemical composition and biological activity of the herbal drug Myrtilli fructus siccus of Croatian origin.

• Keywords: borovnica; suhi plod borovnice; Myrtilli fructus siccus; polifenoli; antocijani; antioksidacijska aktivnost
• Keywords (english): wild bilberry; wild bilberry dry fruit; Myrtilli fructus siccus; polyphenols; anthocyanins; antioxidant activity
• Language: croatian
• URN:NBN: urn:nbn:hr:163:026980
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2024-09-14
• Created on: 2022-09-15 12:38:21