Ultrazvučna ekstrakcija polifenolnih spojeva iz vrste Helichrysum italicum (Roth) G. Don upotrebom (2-hidroksipropil)-β-ciklodekstrina

master's thesis

• Title (english): Ultrasonic extraction of polyphenolic compounds from species Helichrysum italicum (Roth) G. Don. using (2-hydroxypropyl)-B-cyclodextrins
• Author: Dora Tomić
• Mentor: Marijana Zovko Končić (mentor)
• Committee member: Marijana Zovko Končić (predsjednik povjerenstva)
• Committee member: Jasna Jablan (član povjerenstva)
• Committee member: Marijan Marijan (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy); Zagreb
• Defense date and country: 2022-11-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Smilje (Helichrysum italicum, (Roth), G. Don) je izvor visokovrijednih polifenola, prirodnih antioksidansa. Raznolikost tih kemijskih spojeva, koji pojedinačno ili u sinergiji pridonose farmakološkoj aktivnosti, nudi brojne mogućnosti praktične primjene ekstrakata biljke. U cilju učinkovitog izdvajanja navedenih spojeva iz biljnog materijala provedena je optimizacija ekstrakcijskih uvjeta. U radu je korištena metoda inkapsulacije (2-hidroksipropil)-β-ciklodekstrinom, te ultrazvukom potpomognuta ekstrakcija, u svrhu boljeg iskorištenja i skraćenog vremena ekstrakcije. U usporedbi sa konvencionalnom metodom ekstrakcije etanolom ili drugim organskim otapalima, ova metoda je ekološki prihvatljiva. Provedena je spektrofotometrijska analiza sadržaja ukupnih polifenola i podskupina polifenola, fenolnih kiselina i flavonoida u ekstraktima, te kvantifikacija sadržaja klorogenske kiseline korištenjem tekućinske kromatografije visoke djelotvornosti (engl. HPLC). Najboljim uvjetima ekstrakcije pokazali su se neosporno: snaga ultrazvuka 720 W, temperatura 70 °C, masa droge 0,6 g te voda kao otapalo. Parametri s najvećim utjecajem na ekstrakcijski kapacitet su temperatura i masa droge. Snaga ultrazvuka indirektno utječe na prinos dok glicerol gotovo nema utjecaja. Udio mliječne kiseline pozitivno utječe na ekstrakciju fenolnih kiselina pri nižim temperaturama, dok na koncentraciju flavonoida ima negativan utjecaj.

Abstract (english)

Immortelle (Helichrysum italicum, (Roth), G. Don) is a source of high-value polyphenols, natural antioxidants. The diversity of these chemical compounds, which individually or synergistically contribute to pharmacological activity, offers many possibilities in practical use of plant extracts. In order to efficiently extract mentioned compounds from plant material, extraction conditions were optimised. In this paper, methods that were used were (2-hydroxypropyl)-β-cyclodextrin encapsulation and ultrasound-assisted extraction for the purpose of better extraction efficiency and shortened extraction time. In comparison to conventional extraction method with ethanol or other organic solvents, this method is environmentally friendly. Spectrophotometric analysis of total polyphenols, phenolic subclasses, phenolic acids and flavonoids in the extracts was conducted, as well as quantification of chlorogenic acid content using high performance liquid chromatography (HPLC). The best extraction conditions indisputably proved to be: ultrasound power 720 W, temperature 70 °C, drug weight 0,6 g and water as a solvent. Parameters with the greatest impact on the extraction capacity are temperature and drug mass. Ultrasound power affects the yield indirectly, while glycerol had almost no effect. The proportion of lactic acid has a positive effect on phenolic acids’ extraction at lower temperatures, while it has a negative effect on the flavonoid concentration.

• Keywords: smilje; (2-hidroksipropil)-β-ciklodekstrin; ultrazvukom potpomognuta ekstrakcija; polifenoli; flavonoidi; fenolne kiseline; klorogenska kiselina
• Keywords (english): immortelle; (2-hydroxypropyl)-β-cyclodextrins; ultrasound-assisted extraction; polyphenols; flavonoids; phenolic acids; chlorogenic acid
• Language: croatian
• URN:NBN: urn:nbn:hr:163:900650
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2024-11-29
• Created on: 2022-11-30 15:03:34