Utjecaj eteričnog ulja biljne vrste Satureja montana L. i timola na proizvodnju piocijanina bakterije Pseudomonas aeruginosa

master's thesis

• Title (english): The effect of Satureja montana L. essential oil and thymol on pyocyanin synthesis of Pseudomonas aeruginosa
• Author: Filip Sakoman
• Mentor: Gordana Maravić Vlahoviček (mentor)
• Committee member: Gordana Maravić Vlahoviček (predsjednik povjerenstva)
• Committee member: Daniela Jakšić (član povjerenstva)
• Committee member: Maja Bival Štefan (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of biochemistry and molecular biology); Zagreb
• Defense date and country: 2023-02-23, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Pseudomonas aeruginosa ubikvitaran je oportunistički patogen te jedan od najznačajnijih uzročnika bolničkih infekcija. Posjeduje velik broj faktora virulencije između kojih se ističe plavo-zeleni pigment piocijanin koji djeluje proupalno, dovodi do stvaranja slobodnih radikala i oksidativnog stresa te potiče stvaranje biofilma i apoptozu neutrofila. Zbog velike stope morbiditeta i mortaliteta te sve veće antibičke rezistencije uključen je u kategoriju kritičnih bakterijskih patogena za koju je nužno potrebno istraživanje i razvoj novih oblika terapije. Eterična ulja su još iz davnina poznata za svoja antimikrobna svojstva, a eterično ulje biljne vrste Satureja montana L. pokazalo je iznimno antibakterijsko djelovanje te je stoga u ovom radu ispitivan utjecaj eteričnog ulja satureje i njezine glavne monoterpenske sastavnice timola u koncentracijama 0,5 mg/mL, zasebno i u kombinaciji s aminoglikozidnim antibiotikom tobramicinom u koncentraciji 0,5 mg/L , na rast i stvaranje piocijanina bakterijskog soja P. aeruginosa PAO1. Ispitivanje utjecaja na rast bakterijske kulture provedeno je mjerenjem optičke gustoće na 600 nm dok je učinak na proizvodnju piocijanina određen mjerenjem apsorbancije ekstrahiranog piocijanina na 520 nm te normaliziranjem vrijednosti na broj bakterijskih stanica. Rezultati su pokazali da eterično ulje satureje i timol pokazuju inhibitorni učinak na rast bakterijskih stanica P. aeruginosa te sinergistički djeluju na smanjenje rasta u kombinaciji s tobramicinom. S druge strane, timol nije pokazao značajan utjecaj na stvaranje pigmenta piocijanina dok je u prisustvu eteričnog ulja satureje došlo do povećanog stvaranja pigmenta u odnosu na netretiranu bakterijsku kulturu. U kombinaciji s tobramicinom, eterično ulje i timol pokazali su antagonističko djelovanje te doveli do povećanog stvaranja pigmenta. Rezultati ovog rada doprinose boljem razumijevanju kompleksnih interakcija između eteričnih ulja i njezinih sastavnica na P. aeruginosa s ciljem unaprjeđenja terapije infekcija uzrokovanih P. aeruginosa.

Abstract (english)

Pseudomonas aeruginosa is a ubiquitous opportunistic pathogen and one of the most significant causes of healthcare-associated infections. It possesses a variety of virulence factors, namely the blue-green pigment pyocyanin, which acts proinflammatory, leads to the production of free radicals and oxidative stress as well as induces biofilm formation and neutrophil apoptosis. Due to the huge morbidity and mortality and the increasing antibiotic resistance, it is considered a critical pathogen for which research and new therapy are urgently needed. Essential oils have been recognised for their antimicrobial properties, and the essential oil from Satureja montana L. has shown exceptional antibacterial properties. This research examined the effect of Satureja essential oil and its main monoterpene component thymol, both in concentrations 0,5 mg/mL, alone and in the combination with the aminoglycoside antibiotic tobramycin, on the growth and pyocyanin production of the bacterial cells of P. aeruginosa PAO1.
The effect on bacterial growth was examined by measuring the optical density at 600 nm while the effect on pyocyanin production was determined by measuring the absorption of the extracted pyocyanin at 520 nm and normalised on the number of bacterial cells. The results showed that Satureja essential oil and thymol both showed inhibitory effects on P. aeruginosa growth and they both acted synergistically with tobramycin. On the other hand, thymol showed no significant effect on pyocyanin production, while pyocyanin production was increased in the presence of Satureja essential oil. In combination with tobramycin, the essential oil and thymol showed antagonistic action that led to increased pigment production. The results of this study contribute to a better understanding of the complex interaction between essential oils and their components on P. aeruginosa with the goal of improving the therapy of infections caused by P. aeruginosa.

• Keywords: Pseudomonas aeruginosa; Satureja montana L.; eterično ulje; timol; piocijanin; tobramicin; bakterijska međustanična komunikacija
• Keywords (english): Pseudomonas aeruginosa; Satureja montana L.; essential oil; thymol; pyocyanin; tobramycin; quorum sensing
• Language: croatian
• URN:NBN: urn:nbn:hr:163:693605
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-02-23 12:53:25