Učinak visokointenzivne tjelovježbe na plazmatsku koncentraciju galektina-3

master's thesis

• Title (english): Effect of high-intensity exercise on galectin-3 plasma concentration
• Author: Magdalena Vlajčić
• Mentor: Sanja Dabelić (mentor)
• Committee member: Sanja Dabelić (predsjednik povjerenstva)
• Committee member: Sandra Šupraha-Goreta (član povjerenstva)
• Committee member: Erim Bešić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of biochemistry and molecular biology); Zagreb
• Defense date and country: 2023-03-02, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Galektin-3, lektin koji prepoznaje i veže β-galaktozidne jedinice glikokonjugata, može biti smješten u različitim dijelovima stanice ili izvan nje, eksprimiran u brojim tkivima te sudjelovati u raznovrsnim procesima u organizmu. Odnedavno je prepoznat i kao biomarker određenih patoloških stanja. Rezultati dosadašnjih malobrojnih istraživanja o učinku fizičke aktivnosti na plazmatske koncentracije galektina-3 nisu jednoznačni. U ovom radu istražen je učinak kontinuirane visokointenzivne tjelovježbe provedene tijekom šestotjednih progresivno intenzivnijih ponavljanih sprinterskih treninga na mladim i zdravim muškarcima. Uzorci krvi uzimani su u tri vremenske točke: a - prije početka eksperimentalnog protokola, b - neposredno nakon predzadnjeg i najintenzivnijeg treninga te c - mjesec dana nakon završetka protokola. Koncentracije galektina-3 izmjerene su metodom „sendvič“ ELISA, primjenom komercijalno dostupnog kita Quantikine® ELISA Human Galectin-3 Immunoassay. Statistička analiza podataka provedena je dvosmjernom analizom varijance ponovljenih mjerenja u programu GraphPad Prism 9.0.0. Niti u jednoj od tri ispitane vremenske točke, intenzivna tjelovježba nije uzrokovala statistički značajne razlike u plazmatskoj koncentraciji galektina-3 između eksperimentalne i kontrolne skupine. U obje skupine uočen je sličan obrazac smanjenja koncentracije galektina-3, posebice u posljednjoj točki mjerenja (1 mjesec nakon završetka protokola) u odnosu na prvu točku (prije početka protokola). Uzrok takvog ishoda u ovom je istraživanju nepoznat, međutim, s obzirom da su i kontrolnu i eksperimentalnu skupinu činili studenti Kineziološkog fakulteta te da je tijekom istraživanja svima bila dozvoljena tjelovježba u sklopu studija, moguće je da je ta tjelovježba, koja bi se mogla klasificirati kao umjerena do intenzivna, već sama bila dostatna za postizanje uočenog učinka.

Abstract (english)

Galectin-3, a β-galactoside-binding lectin, is localised within various parts of the cell and outside of
it, expressed in multiple tissues and participates in various processes within the organism. Galectin-3 has
recently been recognized as a biomarker of certain pathological states. The results of the few studies done
on the effect of physical activity on plasma concentrations of galectin-3 are not in unison. In this study, the
effect of high-intesity interval training, organised as repeated sprint training with a progressive increase in
intesity in duration of six weeks, has been investigated. The study subjects were young healthy males. The
blood samples were taken at three different time points: a – before the start of the experimental protocol, b –
immediately after the penultimate and most intense training session and c – 1 month after the end of the
experimental protocol. Galectin-3 concentrations were measured by a „sandwich“ ELISA method, using a
commercially available Quantikine® ELISA Human Galectin-3 Immunoassay kit. The statistical data
analysis was done by a two-way analysis of variance in GraphPad Prism 9.0.0. In none of the three
examined time points, intense exercise did not cause statistically significant differences in the plasma
concentration of galectin-3 between the experimental and control groups. In both groups, a similar pattern
of reduction in galectin-3 concentration was observed, especially in the last measurement point (1 month
after the end of the protocol) compared to the first (before the start of the protocol). The cause of such an
outcome in this research is unknown, however, given that both the control and experimental groups were
students of the Faculty of Kinesiology and that during the investigation everyone was allowed to exercise as
part of their studies, it is possible that this exercise, which could be classified as moderate to intense, alone
was sufficient to achieve the observed effect.

• Keywords: galektin-3; visokointenzivna intervalna tjelovježba; plazmatska koncentracija; ELISA
• Keywords (english): galectin-3; high-intensity interval training; plasma concentration; ELISA
• Language: croatian
• URN:NBN: urn:nbn:hr:163:848751
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-03-08 14:27:21