Ultrazvučna ekstrakcija polifenolnih spojeva iz vrste Satureja montana L. upotrebom (2- hidroksipropil)-β-ciklodekstrina i glicerola

master's thesis

• Title (english): Ultrasonic extraction of polyphenolic compounds from Satureja montana L. using (2- hydroxypropyl)-β-cyclodextrin and glycerol
• Author: Helena Margetić
• Mentor: Marijana Zovko Končić (mentor)
• Committee member: Marijana Zovko Končić (predsjednik povjerenstva)
• Committee member: Maja Bival Štefan (član povjerenstva)
• Committee member: Lidija Bach-Rojecky (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy); Zagreb
• Defense date and country: 2023-03-04, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Satureja montana L. (Lamiaceae) sadrži raznolike bioaktivne spojeve, a najviše njih spada u skupinu
polifenolnih spojeva. Zadatak rada bio je ultrazvučnom ekstrakcijom prirediti ekstrakte nadzemih dijelova
vrste S. montana uz upotrebu glicerola i (2-hidroksipropil)-β-ciklodekstrina (HP-β-CD) bogate fenolnim
spojevima: ukupnim polifenolima, fenolnim kiselinama, flavonoidima i ružmarinskom kiselinom. Od 20
ispitivanih uzoraka E10 se istaknuo kao ekstrakt kod kojeg se optimizacijom uvjeta postiglo najbolje
ekstrakcijsko iskorištenje. Sadržaj ukupnih polifenola u tom ekstraktu bio je 5506,42 μg/mL, ukupnih
fenolnih kiselina 3514,44 μg/mL, ukupnih flavonoida 645,72 μg/mL a koncentracija ružmarinske kiseline
822,70 μg/mL. Radom je dokazano kako ekstrakcijski medij voda/glicerol/ HP-β-CD izvrsno ekstrakcijsko
sredstvo za polifenolne spojeve vrste S. montana.

Abstract (english)

Satureja montana L. (Lamiaceae) contains a variety of bioactive compounds, and most of them belong to the group of
polyphenolic compounds. The task of the work was to prepare extracts of the aerial parts of S. montana with the use of
glycerol and (2-hydroxypropyl)-β-cyclodextrin (HP-β-CD) rich in phenolic compounds: total polyphenols, phenolic
acids, flavonoids and rosmarinic acid. Out of 20 tested samples, E10 was the extract in which the optimization of
conditions lead to the highest extraction yield. The content of total polyphenols in this extract was 5506.42 μg/mL, total
phenolic acids was 3514.44 μg/mL, total flavonoids was 645.72 μg/mL and the concentration of rosemarinic acid was
822.70 μg/mL. This work has proven that the water/glycerol/ HP-β-CD is an excellent extraction medium for
polyphenolic compounds of the S. montana.

• Keywords: glicerol; (2-hidroksipropil)-β-ciklodekstrin; polifenoli; ružmarinska kiselina; Satureja montana
• Keywords (english): glycerol; (2-hydroxypropyl)-β-cyclodextrin; polyphenols; rosmarinic acid; Satureja montana L.
• Language: croatian
• URN:NBN: urn:nbn:hr:163:856284
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-04-03
• Created on: 2023-04-13 12:39:42