Utjecaj 2-metoksi-1,4-naftokinona na klijavost bijele gorušice (Sinapis alba L.) i rotkvice (Raphanus sativus L.)

master's thesis

• Title (english): Effect of 2-methoxy-1, 4-naphthoquinone on germination of white mustard (Sinapis alba L.) and radish (Raphanus sativus L.)
• Author: Ana Fajdetić
• Mentor: Ana-Marija Domijan (mentor)
• Mentor: Valerija Vujčić Bok (mentor)
• Committee member: Ana-Marija Domijan (predsjednik povjerenstva)
• Committee member: Valerija Vujčić Bok (član povjerenstva)
• Committee member: Suzana Inić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical botany); Zagreb
• Defense date and country: 2023-03-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

2-metoksi-1,4-naftokinon (2-MNQ) je prirodni derivat 1,4-naftokinona koji je u dosad provedenim ispitivanjima pokazao antipruritično, protuupalno, antialergijsko, antimikrobno i antikancerogeno djelovanje. U ovom istraživanju ispitan je utjecaj 2-MNQ na klijanje sjemenki i razinu oksidacijskog stresa u klijanaca. Kao biljni model korištene su sjemenke dviju biljnih vrsta: bijele gorušice (Sinapis alba L.) i rotkvice (Raphanus sativus L.). Sjemenke su bile izložene 2-MNQ u koncentracijama 1, 5, 10, 20, 30 i 40 μg/ml kroz 3 dana. U pokus su bile uključene negativna kontrola (sjemenke izložene 3 dana destiliranoj vodi) i pozitivna kontrola (sjemenke izložene 3 dana 0,02M otopini CuSO₄). Nakon 3-dnevne izloženosti, za obje biljne vrste određena je klijavost (%) i duljina korjenčića (mm) te je u homogenatu klijanaca određena katalitička aktivnost SOD (U/ml). Podaci su statistički obrađeni pomoću jednosmjerne analize varijance (ANOVA test) te primjenom Duncan's New Multiple Range Test-a. Također, određena je korelacija između mjerenih parametara te analiza glavnih komponenti. Statistički značajnim smatrane su vrijednosti koje se razlikuju na razini p 0,05. Povećanjem koncentracije 2-MNQ opao je broj proklijalih sjemenki bijele gorušice i sukladno tome padala je duljina korjenčića, a 2-MNQ u koncentraciji 30 i 40 μg/ml u potpunosti je inhibirao klijavost sjemenki. To ukazuje da 2-MNQ inhibira klijanje sjemenki bijele gorušice. S druge strane 2-MNQ nije imao utjecaja na klijanje rotkvice. U klijanaca bijele gorušice s koncentracijom 2-MNQ rasla je aktivnost SOD-a te je najveća aktivnost SOD-a izmjerena pri koncentracijama 2-MNQ od 1 i 10 μg/ml. Iz navedenog se može zaključiti kako je biljka tada bila izložena najvećem oksidacijskom stresu te je bilo potrebno proizvesti veliku količinu SOD-a kako bi se od njega obranila i samim time omogućila sjemenkama da prokliju. Katalitička aktivnost SOD-a u rotkvici pri svim koncentracijama otopine 2-MNQ približno je bila ista te nisu uočena odstupanja. Utjecaj 2-MNQ na klijavost sjemenki je različit te ovisi i o koncentraciji 2-MNQ i o samoj biljnoj vrsti. Bijela gorušica osjetljivija je na djelovanje 2-MNQ, dok 2-MNQ u ispitivanim koncentracijama nije imao utjecaja na klijavost rotkvice. Mehanizam toksičnog učinka 2-MNQ može se povezati s oksidacijskim stresom s obzirom da je tretman s 2-MNQ doveo do promjene aktivnosti SOD-a u bijeloj gorušici.

Abstract (english)

2-metoxy-1,4-naphtoquinone (2-MNQ) is a natural derivate of 1,4-naphthoquinone which has shown antipruritic, anti-inflammatory, anti-allergic, antimicrobial and anti-cancer effects.In this study, the influence of 2-MNQ on seed germination and the level of oxidative stress was investigated. As a plant model the seeds of two plant species were used: white mustard (Sinapis alba L.) and radish (Raphanus sativus L.). The seeds were exposed to 2-MNQ in concentrations of 1, 5, 10, 20, 30 and 40 μg/ml for 3 days. The study included a negative control (seeds exposed to distilled water for 3 days) and a positive control (seeds exposed to 0.02M CuSO₄ solution for 3 days). After a 3-day exposure, germination (%) and root length (mm) were measured, and in seedlings homogenate catalytic activity of SOD (U/ml) was determined. Data were statistically processed using one-way analysis of variance (ANOVA test) and Duncan's New Multiple Range Test as well as tested for correlation and Principal Component Analysis. Significance level was set at p ≤ 0.05. With the increase of 2-MNQ concentration the number of germinated seeds of white mustard decreased and 2-MNQ in concentrations of 30 and 40 μg/ml completely ceased germination of white mustard. In parallel the decrease in the length of the radicle with the increase of 2-MNQ concentration was recorded. Thus, 2-MNQ inhibits the germination of white mustard seeds. On contrary, 2-MNQ had no impact on germination of radish. In white mustard seedlings with the increase of 2-MNQ concentration the increase of SOD activity is observed, and the highest SOD activity is recorded at 2-MNQ concentrations 1 and 10 μg/ml. Therefore, it can be concluded that the plant was then exposed to the greatest oxidative stress, and it was necessary to produce a large amount of SOD in order to defend against it and at the same time enable germination. In radish seedlings the catalytic activity of SOD at all concentrations of the 2-MNQ was approximately the same, with no deviations. The impact of 2-MNQ on the seed germination is different and depends on the concentration of 2-MNQ and the plant species itself. White mustard is more sensitive to 2-MNQ, while 2-MNQ in concentrations tested had no impact on radish. The mechanism of the toxic effect of 2-MNQ can be related to oxidative stress, considering that treatment with 2-MNQ led to a change in SOD activity in white mustard.

• Keywords: 2-MNQ; bijela gorušica; rotkvica; klijavost; duljina korjenčića; oksidacijski stres
• Keywords (english): 2-MNQ; white mustard; radish; germination; root lenght; oksidacijski stres
• Language: croatian
• URN:NBN: urn:nbn:hr:163:425390
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-04-13 12:51:43