Biološka aktivnost glicerolnih ekstrakata širokolisnog trputca (Plantago major L.) bogatih fenolnim kiselinama i aukubinom za primjenu u kozmetici

master's thesis

• Title (english): Biological activity of glycerol extracts of plantain (Plantago major L.) rich in phenolic acids and aucubin for use in cosmetics
• Author: Adriana Ninić
• Mentor: Marijana Zovko Končić (mentor)
• Committee member: Marijana Zovko Končić (predsjednik povjerenstva)
• Committee member: Maja Bival Štefan (član povjerenstva)
• Committee member: Lidija Bach-Rojecky (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy); Zagreb
• Defense date and country: 2023-06-16, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Biljni ekstrakti koji sadrže polifenolne i iridoidne sastavnice imaju širok spektar djelovanja zbog
čega su zanimljivi farmaceutskoj, ali i kozmetičkoj industriji. Veliki trputac (Plantago major L.) je
ljekovita biljka koja od davnina ima bogatu terapijsku primjenu. Cilj ovog istraživanja bio je ispitati
antioksidativni i biološki učinak glicerolnih ekstrakata ove biljke kako bi se utvrdio potencijal
njihove primjene u pripravcima protiv starenja kože. Antioksidativna aktivnost ekstrakata ispitana
je 2,2-difenil-1-pikrilhidrazil (DPPH) metodom dok je biološka aktivnost ispitana testovima
inhibicije enzima elastaze, tirozinaze, kolagenaze i hijaluronidaze. Ekstrakti su optimizirani na
ukupne fenolne kiseline (OPT-TPA) i aukubin (OPT-AUC), a rezultati ispitivanja su izraženi kao
IC50 vrijednost. Oba ekstrakta pokazala su bolju sposobnost hvatanja slobodnih radikala i inhibicije
kolagenaze od pozitivnih kontrola. Također, ostvarili su značajnu inhibiciju hijaluronidaze i
umjerenu inhibiciju tirozinaze. Ekstrakt OPT-AUC se istaknuo kao dva puta bolji inhibitor elastaze
od ekstrakta OPT-TPA. S obzirom da su ispitivanja provedena na in vitro modelu potrebno je
provesti daljnja klinička istraživanja kako bi se potvrdio utjecaj ekstrakata na starenje kože.

Abstract (english)

Plant extracts containing polyphenolic and iridoid components have a wide spectrum of action,
which is why they are interesting for the pharmaceutical and cosmetic industries. Plantain (Plantago
major L.) is a medicinal plant that has had a rich therapeutic application since ancient times. The
aim of this research was to examine the antioxidant and biological effect of glycerol extracts of this
plant in order to determine the potential of their use in antiaging skin products. The antioxidant
activity of the extracts was tested with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, while the
biological activity was tested with elastase, tyrosinase, collagenase and hyaluronidase enzyme
inhibition tests. Extracts were optimized for total phenolic acids (OPT-TPA) and aucubin (OPTAUC),
and test results were expressed as IC50 value. Both extracts showed a better ability to
scavenge free radicals and inhibit collagenase than the positive controls. They also achieved
significant inhibition of hyaluronidase and moderate inhibition of tyrosinase. OPT-AUC extract
stood out as two times better elastase inhibitor than OPT-TPA extract. Given that the tests were
conducted on an in vitro model, further clinical research is necessary to confirm the effect of the
extracts on skin aging.

• Keywords: Plantago major; starenje kože; antioksidativni učinak; fenolne kiseline; aukubin; elastaza; tirozinaza; kolagenaza; hijaluronidaza
• Keywords (english): Plantago major; skin aging; antioxidant effect; phenolic acids; aucubin; elastase; tyrosinase; collagenase; hyaluronidase
• Language: croatian
• URN:NBN: urn:nbn:hr:163:970857
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-06-16
• Created on: 2023-11-08 14:03:53