Antioksidativna aktivnost formula za nadomjesnu prehranu

master's thesis

• Title (english): Antioxidant activity of enteral formulas
• Author: Iva Filković
• Mentor: Dubravka Vitali Čepo (mentor)
• Committee member: Dubravka Vitali Čepo (predsjednik povjerenstva)
• Committee member: Kristina Radić (član povjerenstva)
• Committee member: Marija Grdić Rajković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of food chemistry); Zagreb
• Defense date and country: 2023, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Uloga prehrane u prevenciji i liječenju brojnih kroničnih nezaraznih bolesti sve se više prepoznaje
kao ključna za održavanje zdravlja odnosno postizanje optimalnih ishoda liječenja. U tom kontekstu
se uz klasičnu dijetoterapiju sve više primjenjuje i ciljana vitaminsko-mineralna suplementacija, a
kod bolesti s izraženom malnutricijom, i enteralne formule optimiranog nutritivnog sastava. Osim
izbalansiranog sastava esencijalnih makro- i mikronutrijenata nove generacije enteralnih formulacija
vrlo često sadrže i neesencijalne sastavnice dokazanih bioloških učinaka, najčešće iz skupina spojeva
s antioksidativnim učinkom. U ovom radu istražen je učinak modifikacije standardne enteralne
formulacije dodatkom funkcionalnih sastavnica s obzirom na sadržaj polifenola i drugih spojeva s
antioksidacijskom aktivnošću. Ukupni sadržaj polifenola određen je Folin-Ciocalteu metodom
nakon odmašćivanja i uklanjanja interferirajućih proteina Carez reagensom. Antioksidacijski
potencijal određen je spektrofotometrijski u istim uzorcima, TEAC metodom. Dobiveni rezultati
pokazali su da se dodatkom resveratrola u formulaciju, kao i zamjenom dijela sirovina cjelovitim
žitaricama značajno povećava antioksidativna aktivnost uzoraka te da se upravo polifenoli mogu
smatrati glavnim nositeljima antioksidativne aktivnosti analiziranih proizvoda. Utvrđeno je da se
skladištenjem analiziranih proizvoda na sobnoj temperaturi ukupni antioksidacijski potencijal malo,
ali statistički značajno smanjuje u uzorcima s dodatkom polifenola masline i resveratrola. Dobiveni
rezultati doprinijeti će razumijevanju trenutnih mogućnosti i ograničenja u razvoju inovativnih
formulacija za enteralnu primjenu.

Abstract (english)

The role of nutrition in the prevention and treatment of numerous chronic indistinct diseases is
increasingly being recognized as key to maintaining health, i.e. achieving optimal treatment
outcomes. In this context, in addition to classical diet therapy, targeted vitamin and mineral
supplementation is increasingly used, and in diseases with severe malnutrition, enteral formulas with
an optimized nutritional composition. In addition to the balanced composition of essential macroand micronutrients, the new generation of enteral formulations very often contain non-essential
components with proven biological effects, most often from groups of compounds with antioxidant
effects. This paper investigated the effect of modifying the standard enteral formulation with the
addition of functional components with regard to the content of polyphenols and other compounds
with antioxidant activity. The total content of polyphenols was determined by the Folin-Ciocalteu
method in defatted samples, after removing interfering proteins with the Carez reagent. The
antioxidant potential was determined spectrophotometrically in the same samples, using the TEAC
method. The obtained results showed that the addition of resveratrol to the formulation, as well as
the replacement of part of the raw materials with whole grains, significantly increases the antioxidant
activity of the samples and that polyphenols can be considered the main carriers of the antioxidant
activity of the analyzed products. It was found that by storing the analyzed products at room
temperature, the total antioxidant potential decreases slightly, but statistically significantly in
samples enriched with olive-polyphenols and resveratrol. The obtained results will contribute to the
understanding of current opportunities and limitations in the development of innovative
formulations for enteral administration.

• Keywords: antioksidans; polifenoli; enteralne formule; reservatrol; maslina; bijelo brašno; integralne žitarice
• Keywords (english): antioxidant; polyphenols; enteral formulas; reservatrol; olive; white flour; whole grains
• Language: croatian
• URN:NBN: urn:nbn:hr:163:448089
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-07-10
• Created on: 2023-11-16 11:57:49