Spektrofotometrijsko određivanje ukupnih polifenola i antioksidativne aktivnosti dodataka prehrani za liječenje upalnih bolesti crijeva

master's thesis

• Title (english): Spectrophotometric determination of total polyphenols and antioxidant activity of dietary supplements for the treatment of inflammatory bowel diseases
• Author: Matea Klobučar
• Mentor: Daniela Amidžić Klarić (mentor)
• Committee member: Daniela Amidžić Klarić (predsjednik povjerenstva)
• Committee member: Maja Ortner Hadžiabdić (član povjerenstva)
• Committee member: Goran Poje (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical analysis); Zagreb
• Defense date and country: 2023-07-13, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Upalne bolesti crijeva, Chronova bolest i ulcerozni kolitis, kronične su relapsno-remitirajuće bolesti
koje postaju sve češće te narušavaju kvalitetu života mnogih ljudi. Mnogi se, uz farmakoterapiju,
okreću alternativnoj medicini i ekstraktima biljaka koje imaju protuupalno i antioksidativno
djelovanje zahvaljujući polifenolima koje sadrže. Cilj ovog diplomskog rada bio je procijeniti
kvalitetu pribavljenih dodataka prehrani za liječenje upalnih bolesti crijeva s obzirom na njihov
sadržaj ukupnih polifenola i antioksidativne aktivnosti. Sadržaj ukupnih polifenola određen je
spektrofotometrijski korištenjem Folin-Ciocalteu reagensa, a antioksidativna aktivnost procijenjena
je ABTS metodom. Sadržaj ukupnih polifenola monopreparata kurkume bio je veći nego u ostalih
analiziranih preparata. Najveća antioksidativna aktivnost bila je kod monopreparata piperina.
Ispitivani uzorci koji sadrže kurkumu, indijski tamjanovac, justiciju i crni papar mogu se smatrati
dobrim izvorom polifenolnih sastavnica.

Abstract (english)

Inflammatory bowel diseases, Crohn's disease and ulcerative colitis are chronic relapsing-remitting
diseases that are becoming increasingly common and impair the life quality of many people. Many
patients, in addition to pharmacotherapy, turn to alternative medicine and plant extracts that have
anti-inflammatory and antioxidant effects thanks to the polyphenols they contain. This thesis aimed
to evaluate the quality of the obtained nutritional supplements for the treatment of inflammatory
bowel diseases with regard to their content of total polyphenols and antioxidant activity. The content
of total polyphenols was determined spectrophotometrically using the Folin-Ciocalteu reagent, and
the antioxidant activity was assessed using the ABTS method. The content of total polyphenols in
the turmeric monopreparation was higher than in the other analyzed preparations. The highest
antioxidant activity was found in monopreparations of piperine. Tested samples containing turmeric,
Indian frankincense, justice, and black pepper can be considered as a good source of polyphenol
components.

• Keywords: upalne bolesti crijeva; Folin-Ciocalteu metoda; ABTS metoda; Curcuma longa; Boswellia serrata; Piper nigrum; Andrographis paniculata; antioksidansi; ukupni polifenoli
• Keywords (english): inflammatory bowel diseases; Folin-Ciocalteu method; ABTS method; Curcuma longa; Boswellia serrata; Piper nigrum; Andrographis paniculata; antioxidants; total polyphenols
• Language: croatian
• URN:NBN: urn:nbn:hr:163:317035
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-07-13
• Created on: 2023-11-21 14:02:39