Ekspresija gena c-MYC u pacijenata s kolorektalnim karcinomom

master's thesis

• Title (english): c-MYC expression in colorectal cancer patients
• Author: Karmela Mićan
• Mentor: Karmela Barišić (mentor)
• Committee member: Karmela Barišić (predsjednik povjerenstva)
• Committee member: Donatella Verbanac (član povjerenstva)
• Committee member: Ivana Ćelap (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of medical biochemistry and haematology); Zagreb
• Defense date and country: 2023-07-18, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Medical Biochemistry

Abstract

Kolorektalni karcinom (CRC) zbog visoke incidencije i stope smrtnosti predstavlja značajan javnozdravstveni problem.
Ključni korak u borbi protiv ove maligne bolesti podrazumijeva rano postavljanje dijagnoze. Trenutno su mnoga
istraživanja usmjerena na ispitivanje metode tekuće biopsije kao inovativnoga rješenja na području dijagnostike
malignih bolesti. S ciljem uvođenja tekuće biopsije u kliničku praksu, javlja se potreba za određivanjem te validacijom
potencijalnih biljega za ranu dijagnozu, odabir terapije, praćenje uspješnosti terapije, prognozu bolesti te ranu detekciju
recidiva. U ovom radu ispitivao se dijagnostički potencijal gena c-MYC usporedbom ekspresije gena c-MYC iz RNA
izolirane iz tkiva u bolesnika s adenomom (pre-CRC) te bolesnika s CRC-om. U istraživanju je sudjelovalo 46 ispitanika
od kojih je 33 ispitanika s pre-CRC-om te 13 ispitanika s potvrđenim CRC-om. Rezultati analize nisu potvrdili potencijal
gena c-MYC kao biljega za rano otkrivanje CRC-a budući da nije postojala statistički značajna razlika između ekspresije
gena c-MYC kod pacijenata s pre-CRC-om te potvrđenim CRC-om (p > 0,05). Istovremeno, u istraživanju se ispitivao
potencijal gena c-MYC kao biljega za rano otkrivanje CRC-a primjenom metode tekuće biopsije kod 18 pacijenata s
potvrđenim CRC-om. U uzorcima RNA izoliranih iz egzosoma nije detektirana ekspresija ni ciljnoga (c-MYC) niti
referentnog gena (GAPDH) te stoga nije bilo moguće provesti daljnju usporedbu s rezultatima ekspresije gena c-MYC
iz RNA izolirane iz tkiva bolesnika s pre-CRC-om te potvrđenim CRC-om. Neuspješna detekcija c-MYC te GAPDH
gena može biti posljedica niske koncentracije RNA izolirane iz egzosoma, degradiranosti RNA molekule ili visokog
udjela miRNA molekula u ukupnoj izoliranoj RNA.

Abstract (english)

Colorectal cancer (CRC) is a significant public health problem due to its high incidence and mortality rate. A key step
in the fight against this malignant disease involves early diagnosis. Currently, many studies are focused on testing the
method of liquid biopsy as an innovative solution in the field of diagnosis of malignant diseases. With the aim of
introducing liquid biopsy into clinical practice, there is a need to determine and validate potential markers for early
diagnosis, therapy selection, therapy success monitoring, disease prognosis and early recurrence detection. In this work,
the diagnostic potential of the c-MYC gene was examined by comparing the expression of the c-MYC gene in RNA
isolated from a tissue of patients with adenoma (pre-CRC) and patients with confirmed CRC. 46 subjects participated
in the study, of which 33 subjects had pre-CRC and 13 subjects had confirmed CRC. The results of the analysis did not
confirm the potential of the c-MYC gene as a marker for the early detection of CRC, since there was no statistically
significant difference between the expression of the c-MYC gene of subjects with pre-CRC and confirmed CRC (p >
0.05). At the same time, the research examined the potential of the c-MYC gene as a marker for early detection of CRC
using the liquid biopsy method of 18 subjects with confirmed CRC. Neither the target (c-MYC) nor the reference gene
(GAPDH) expression was detected in RNA samples isolated from exosomes, and therefore it was not possible to carry
out a further comparison with the results of c-MYC gene expression out of RNA isolated from the tissue of patients with
pre-CRC and confirmed CRC. Unsuccessful detection of c-MYC and GAPDH genes may be due to low concentration
of RNA isolated from exosomes, degradation of RNA molecules or a high proportion of miRNA molecules out of total
isolated RNA.

• Keywords: kolorektalni karcinom; tekuća biopsija; egzosomi; RNA; c-MYC; qPCR
• Keywords (english): colorectal cancer; liquid biopsy; exosomes; RNA; c-MYC; qPCR
• Language: croatian
• URN:NBN: urn:nbn:hr:163:489137
• Study programme: Title: Medical biochemistry; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra medicinske biokemije (magistar/magistra medicinske biokemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-11-21 15:02:47